Isolation of Bacteria Sphingobacterium Thalpophilum and Streptococcus Thoraltensis from Oreeochromis Aureus (Steindachner, 1864)) and Antibiotic Susceptibility in Al-Diwaniyah River / Middle Iraq

Abstract

This study was carried out to investigate the occurrence of potentially pathogenic species of bacterium in  O. aureus in Al-Diwaniya River. A total of 100 fishes of  O. aureus, Fish during the period April  2021 till March 2022. Bacteria were identified using the VITEK 2 system and selected biochemical tests. species were identified in a  O. aureus which are S. thoraltensis,  S. thalpophilum. Antibiotic susceptibility test of 16 antibiotics, which are (Ampicillin, piperacillin/ tazobactan, amikacin, cefazolin, Ceftriaxone, Ertapenem, Gentamicin, cefoxitin, ciprofloxacin, Imipenem, ceftazidime, levofloxacin, cefepime, tigecycline, Nitrofurantion, and trimethoprim/ sulfamethoxazoleby) were identified and examined for antibiotic susceptibility using the Vitek II system. The results indicate that the bacteria sensitive to Minimum Inhibitory  antibiotics susceptibility were Ciprofloxacin(<= 0. 23, <= 0. 25 μg/ ml), on S. thoraltensis,   and S. thalpophilum. Imipenem and Levofloxacin (<= 0. 20, <= 0. 25 μg/ ml), when tested in vitro on S. thoraltensis, S. thalpophilum, while it was resistant to Cefoxitin(<= 66, <= 70 μg/ ml) on S. thoraltensis,   and S. thalpophilum.