Biosynthesis Of Ethanolic Extract Using Clitoria Ternatea And Estimation Of Antioxidant Capacity
Keywords:
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Abstract
As it changes colour toward blue, Clitoria ternatea or butterfly pea’s reproductive blossom is admired and valued highly. Like the rest of the family Fabaceae, this plant too has 58 species which are known to occur in a globe shape 1. This Perennial herb is also cultivated in cultural and medicinal aspect in places like Bengal and India. “Blue” tea, renowned internationally, derives its name from the butterfly pea plants sepal and as blue tea’s colour also changes in accordance with the acidity of the medium—hypothetically shifts during infusion to alkaline water and citric acid. When citric acid is added to the tea pot, it indeed turns violet which suggests acidity 2.
References
1. Oguis GK, Gilding EK, Jackson MA, Craik DJ. Butterfly Pea (Clitoria ternatea), a Cyclotide-Bearing Plant With Applications in Agriculture and Medicine [Internet]. Front Plant Sci. 2019;10:645. Available from: http://dx.doi.org/10.3389/fpls.2019.00645
2. Gamage GCV, Lim YY, Choo WS. Anthocyanins From Clitoria ternatea Flower: Biosynthesis, Extraction, Stability, Antioxidant Activity, and Applications [Internet]. Front Plant Sci. 2021;12:792303. Available from: http://dx.doi.org/10.3389/fpls.2021.792303
3. Zafra-Stone S, Yasmin T, Bagchi M, Chatterjee A, Vinson JA, Bagchi D. Berry anthocyanins as novel antioxidants in human health and disease prevention. Mol Nutr Food Res. 2007;51(6):675–83.
4. Jeyaraj EJ, Lim YY, Choo WS. Extraction methods of butterfly pea (Clitoria ternatea) flower and biological activities of its phytochemicals [Internet]. J Food Sci Technol. 2021;58:2054–67. Available from: http://dx.doi.org/10.1007/s13197-020-04745-3
5. Maneesai P, Iampanichakul M, Chaihongsa N, Poasakate A, Potue P, Rattanakanokchai S, et al. Butterfly Pea Flower (Clitoria ternatea Linn.) Extract Ameliorates Cardiovascular Dysfunction and Oxidative Stress in Nitric Oxide-Deficient Hypertensive Rats [Internet]. Antioxidants. 2021;10(4):523. Available from: http://dx.doi.org/10.3390/antiox10040523
6. Nair V, Bang WY, Schreckinger E, Andarwulan N, Cisneros-Zevallos L. Protective Role of Ternatin Anthocyanins and Quercetin Glycosides from Butterfly Pea (Clitoria ternatea Leguminosae) Blue Flower Petals against Lipopolysaccharide (LPS)-Induced Inflammation in Macrophage Cells. J Agric Food Chem. 2015;63(28):6355–65.
7. Escher GB, Wen M, Zhang L, Rosso ND, Granato D. Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals [Internet]. Food Chem. 2020;331:127341. Available from: http://dx.doi.org/10.1016/j.foodchem.2020.127341
8. Chandra S, Rawat DS. Medicinal plants of the family Fabaceae in India. Pharmacogn Rev. 2013;7(13):53–83.
9. Sunil M, Ramesh R. Antimicrobial, Anti-inflammatory, and Antioxidant Properties of Punica Granatum Peel: An In-Vitro Analysis. Cuestiones de Fisioterapia. 2025;54(3):2572-82.
10. Varshini A, Gurunathan D, Padmapriya S. Prevalence of class I caries in the second mandibular primary molar in 3–6-year-old children. J Adv Pharm Technol Res. 2022;13(Suppl 1):S72-5.
11. Kim JH, Lee BC. Anthocyanins as potent antioxidants in human health. J Food Sci Technol. 2021;58(2):567–74.
12. Khoo HE, Azlan A, Tang ST, Lim SM. Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutr Res. 2017;61:1361779.
13. Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. Sci World J. 2013;2013:162750.
14. Li Y, Yao J, Han C, et al. Quercetin, inflammation and immunity. Nutrients. 2016;8(3):167.
15. Calderón-Montaño JM, Burgos-Morón E, Pérez-Guerrero C, López-Lázaro M. A review on the dietary flavonoid kaempferol. Mini Rev Med Chem. 2011;11(4):298–344.
16. Chumsri P, Sirikanchanarod A, Chantarangkul S, et al. Antioxidant and anti-inflammatory activities of Clitoria ternatea flower extract. Pharm Biol. 2018;56(1):1–8.
17. Rai KS, Murthy KD, Karanth KS, Rao MS. Clitoria ternatea root extract enhances acetylcholine content in rat hippocampus. Fitoterapia. 2002;73(7-8):685–9.
18. Ghosh S, Samanta A, Mandal NB, Pal M. Anti-inflammatory and antioxidant activity of Clitoria ternatea leaf extracts. Asian Pac J Trop Med. 2010;3(6):470–3.
19. Mukherjee PK, Kumar V, Mal M, Houghton PJ. Acetylcholinesterase inhibitors from plants. Phytomedicine. 2007;14(4):289–300.
20. Sharma S, Gupta D, Mishra DP. Nanotechnology in pharmaceutical formulations: Strategies for enhancing bioavailability. Biomed Pharmacother. 2019;112:108755.
2. Gamage GCV, Lim YY, Choo WS. Anthocyanins From Clitoria ternatea Flower: Biosynthesis, Extraction, Stability, Antioxidant Activity, and Applications [Internet]. Front Plant Sci. 2021;12:792303. Available from: http://dx.doi.org/10.3389/fpls.2021.792303
3. Zafra-Stone S, Yasmin T, Bagchi M, Chatterjee A, Vinson JA, Bagchi D. Berry anthocyanins as novel antioxidants in human health and disease prevention. Mol Nutr Food Res. 2007;51(6):675–83.
4. Jeyaraj EJ, Lim YY, Choo WS. Extraction methods of butterfly pea (Clitoria ternatea) flower and biological activities of its phytochemicals [Internet]. J Food Sci Technol. 2021;58:2054–67. Available from: http://dx.doi.org/10.1007/s13197-020-04745-3
5. Maneesai P, Iampanichakul M, Chaihongsa N, Poasakate A, Potue P, Rattanakanokchai S, et al. Butterfly Pea Flower (Clitoria ternatea Linn.) Extract Ameliorates Cardiovascular Dysfunction and Oxidative Stress in Nitric Oxide-Deficient Hypertensive Rats [Internet]. Antioxidants. 2021;10(4):523. Available from: http://dx.doi.org/10.3390/antiox10040523
6. Nair V, Bang WY, Schreckinger E, Andarwulan N, Cisneros-Zevallos L. Protective Role of Ternatin Anthocyanins and Quercetin Glycosides from Butterfly Pea (Clitoria ternatea Leguminosae) Blue Flower Petals against Lipopolysaccharide (LPS)-Induced Inflammation in Macrophage Cells. J Agric Food Chem. 2015;63(28):6355–65.
7. Escher GB, Wen M, Zhang L, Rosso ND, Granato D. Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals [Internet]. Food Chem. 2020;331:127341. Available from: http://dx.doi.org/10.1016/j.foodchem.2020.127341
8. Chandra S, Rawat DS. Medicinal plants of the family Fabaceae in India. Pharmacogn Rev. 2013;7(13):53–83.
9. Sunil M, Ramesh R. Antimicrobial, Anti-inflammatory, and Antioxidant Properties of Punica Granatum Peel: An In-Vitro Analysis. Cuestiones de Fisioterapia. 2025;54(3):2572-82.
10. Varshini A, Gurunathan D, Padmapriya S. Prevalence of class I caries in the second mandibular primary molar in 3–6-year-old children. J Adv Pharm Technol Res. 2022;13(Suppl 1):S72-5.
11. Kim JH, Lee BC. Anthocyanins as potent antioxidants in human health. J Food Sci Technol. 2021;58(2):567–74.
12. Khoo HE, Azlan A, Tang ST, Lim SM. Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutr Res. 2017;61:1361779.
13. Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. Sci World J. 2013;2013:162750.
14. Li Y, Yao J, Han C, et al. Quercetin, inflammation and immunity. Nutrients. 2016;8(3):167.
15. Calderón-Montaño JM, Burgos-Morón E, Pérez-Guerrero C, López-Lázaro M. A review on the dietary flavonoid kaempferol. Mini Rev Med Chem. 2011;11(4):298–344.
16. Chumsri P, Sirikanchanarod A, Chantarangkul S, et al. Antioxidant and anti-inflammatory activities of Clitoria ternatea flower extract. Pharm Biol. 2018;56(1):1–8.
17. Rai KS, Murthy KD, Karanth KS, Rao MS. Clitoria ternatea root extract enhances acetylcholine content in rat hippocampus. Fitoterapia. 2002;73(7-8):685–9.
18. Ghosh S, Samanta A, Mandal NB, Pal M. Anti-inflammatory and antioxidant activity of Clitoria ternatea leaf extracts. Asian Pac J Trop Med. 2010;3(6):470–3.
19. Mukherjee PK, Kumar V, Mal M, Houghton PJ. Acetylcholinesterase inhibitors from plants. Phytomedicine. 2007;14(4):289–300.
20. Sharma S, Gupta D, Mishra DP. Nanotechnology in pharmaceutical formulations: Strategies for enhancing bioavailability. Biomed Pharmacother. 2019;112:108755.
Published
2023-12-16
How to Cite
Praveen Kumar S, Dr. Ramesh R, & Dr. S. Rajeshkumar. (2023). Biosynthesis Of Ethanolic Extract Using Clitoria Ternatea And Estimation Of Antioxidant Capacity. Revista Electronica De Veterinaria, 24(4), 656-660. https://doi.org/10.69980/redvet.v24i4.1906
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