Prevalence and Molecular Studies of Isospora Spp. in House and Stray Cats at Baghdad Province
The purpose of this study was to look at the prevalence of the intestinal protozoa Isospora spp. in cats in Baghdad from November 1st to April 30th, 2018 in terms of sex, age group (Young and Adult), and months. The current study's findings indicated that both sexes were infected with Isospora spp (with non-significant differences). In domestic cats, 3(10.3%) of 29 males were found diseased, whereas 5(5%) of 31 females were afflicted (16.1 percent). In addition, infection rates in stray cats were 8% in 25 males and 14.2% in 35 females. There were no significant differences (P> 0.05) in infection rates between young and adult house cats and stray cats. In house cats, the greatest rate of infection with Isospora spp. (20%) was reported in March and April, with a significant difference (P0.05), whereas in stray cats, the highest rate of infection (20%) was recorded in December, with a significant difference (P0.05). In this investigation, one PCR primer was utilized to identify Isospora utilizing the 18S rDNA ribosomal gene. The increased PCR readings might be related to the discovery of residual DNA from previous infections.