Characterization of Bacteriophage Targeting S. aureus Isolated from Caprine Mastitis

Anil Kumar Mishra; K. Gururaj; Ashish Kumar Churamani; Nitika Sharma

doi:10.69980/redvet.v26i2.2489

Anil Kumar Mishra
K. Gururaj
Ashish Kumar Churamani
Nitika Sharma

DOI: https://doi.org/10.69980/redvet.v26i2.2489

Keywords: Bacteriophage, Staphylococcus aureus, Lytic activity, MOI, EOP, Phage stability, One-step growth curve, Phage therapy.

Abstract

The current study was conducted to characterize phages virulent to Staphylococcus aureus isolates from caprine mastitis as well as from cattle/buffalo to assess their potential in phage therapy. Four bacteriophages were analyzed based on biological properties, namely the lytic range, multiplicity of infection (MOI), efficiency of plating (EOP), resistance to variations in temperature and pH, as well as growth kinetics. Out of four bacteriophages studied, only one isolate (isolate number 1) showed lytic activity, lysing 32 out of 121 isolates of S. aureus with lytic range of 26.44%. The optimal MOI of the phage was calculated to be 1.71, and the phage had a high EOP score of 0.88, which demonstrates high efficiency of infection. The tested phage showed stability at temperature changes between 4 °C and 55 °C and variations in pH of 5–11, showing the highest stability in the close to 37 °C and neutral pH. The latent period for this phage according to the one-step growth analysis was estimated 20 minutes with a burst size of 295 PFU. These findings show that the characterized bacteriophage was having good biological properties suitable for application in therapy but its narrow host range requires further investigations.

Author Biographies

Anil Kumar Mishra

Senior Scientist, Animal Health Division, ICAR- Central Institute for Research on Goats, Makhdoom, Farah, Mathura-281122, U.P.,

K. Gururaj

Principal Scientist, Animal Health Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah-281122, Mathura, U. P.,

Ashish Kumar Churamani

Junior Research Assistant, Animal Health Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah-281122, Mathura, U. P.,

Nitika Sharma

Senior Scientist, Animal Health Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah-281122, Mathura, U. P.,

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