Residual Aflatoxin and Histopathological Changes in Broiler Chickens Amended with Rhino-Hepato ® Forte During Aflatoxicosis
Four groups of broiler chickens have been treated as follows: Group 1 (control group, without AF or RHF; Group 2: (Feed contaminated with 500ppb AF without RHF), G3 (Feed contaminated with 500ppb AF+ 1 ml of RHF in drinking water), G4 (Feed contaminated with 500ppb AF+ 2 ml of RHF in drinking water). ELISA assay was conducted to estimate the AFB1 residual in the liver and the histopathological changes in livers as a result of AFB1 consumption per se or with antidote RHF.
No AFB1 residues were observed in the control group livers of. Residues of 15.18 µg/kg were found in the liver tissues of the group that was fed with 500 ppb of AFB1. Significantly lower residues of 1.14 and 1.012 µg/kg were detected in livers of G3 and G4 respectively compared to AFB1fed group (G2).
feeding broilers with AFB1 at levels of 500 showed measurable AFB1 residues in the tissues of the liver and enhancing mild-moderate to severe histopathological changes. Amending broiler chickens fed AFB1 with RHF significantly ameliorated the AFB1 negative effect on AFB1 residual in the liver content and was able to counteract the histopathological changes of the toxin on liver parenchyma. Therefore, the researcher conducted the study to estimate aflatoxin residues of B1in the liver (AFB1), and histopathological changes in liver via the chronic aflatoxicosis in chicks that are fed with AFB1and Rhino-Hepato ® Forte (RHF).