Histopathological Changes in Testicular Tissues of Male Rabbits by Trimetion

  • Sattar Rashid S. Al-Eqabi Department of Veterinary Public Health, Veterinary Medicine College, University of Wasit, Wasit, Iraq
Keywords: Necrosis, Degeneration, Reproductive system, Iraq


This study was conducted to investigate pathological lesions associated with Trimetion intoxication on testes and epididymis and fertility in adult male rabbits. Totally, 24 local male rabbits were purchased, prepared and randomly divided equally into four groups; (G1) negative control group, (G2) drenched Trimetion at 10 ml/Kg daily for two months; (G3) drenched Trimetion at 25 ml/Kg daily for two months; and (G4) drenched Trimetion at 50 ml/Kg daily for two months using of oral gavage. In contrast to control group, the testes of the treated groups had atrophy. The primary histopathological lesions of drenched rabbits revealed that Trimetion causes a dose-related damage to testes as evidenced by mild hydropic degeneration, sloughing of cell lining and by partial suppression of spermatogenesis in G1; while in G2, epididymis appears to be lack of sperm. In G3, there were more severe testicular damages with little hydropic degeneration, spermatogonial inhibition, and severe lack of spermatogenesis. The findings of G4 had aggressive, severe, and widespread lesions in all testicular tissue, severe necrosis and full spermatogonial suppression with no spermatogonia. In conclusion, the current study showed that the Trimetion is caused different pathological lesions in male testicular and epididymal tissues of adult rabbits which appeared microscopically sever necrosis and degenerations and complete suppression of spermatogenesis with vaculation of spermatogonia. However, moreover experiments are required to identify other toxicopathological changes in other body tissues.


United States Environmental Protection Agency, EPA (2008): Pesticide homepage, http://www.epa.gov/opp00001/.

Kamel, F. and Hoppin, JA. (2004): Association of Pesticide Exposure with Neurologic Dysfunction and Disease. Environmental Health Perspectives. 112(9):950-958.

Mnif, W.; Hassine, AI.;Bouaziz, A.; Bartegi, A.; Thomas, O. and Roig, B. (2011): Effect of Endocrine Disruptor Pesticides: A Review. International Journal of Environmental Research and Public Health, 8(6):2265-2303.

WHO/UNEP. (1990).Public Health Impact of Pesticides Used in Agriculture. Geneva, p. 128.

Hayes, W.J. and E.R. Laws, (1991). Handbook of Pesticide Toxicology. 1st Edn., Academic Press, San Diego, CA., ISBN-10: 0123341604, pp: 1523.

Meister, RT. (1992): Farm chemicals handbook. Willoughby, OH: Meister Publishing Company. Willoghby, OH.

IPCS/WHO, (1996). Principles and Methods for Assessing Direct Immunotoxicity Associated with Exposure to Chemicals. 1st Edn., Geneva, Switzerland.

Rawlings, N.C., Cook, S.J. and Waldbillig, D. (1998). ‘‘Effect of the Pesticides CarbofuranChlorpyrifos, Trimetion, Lindane, Triallates, Trifluralin, 2, 4-D and Entachlorophenol on the Metabolic Endocrine and Reproductive Endocrine System in Ewes.” J. of Toxicol. and Environ. Hlth., 54, No. 10 21- 36.

Abdallah, F.B., A.B. Slima, I. Dammak, L. Keskes- Ammar and Z. Mallek. (2010). Comparative effects of Trimetion and deltamethrin on reproductive system in mice.Andrologia, 42: 182-186. DOI: 10.1111/j.1439-0272.2009.00976.x.

Farag, A.T., T.A.Z. Karkour and A.E. Okazy, (2006). Developmental toxicity of orally administered technical Trimetion in rats. Birth Defects Res., 77: 40-46. PMID: 16496292.

Kaur, S. and C.K. Dhanju, (2005). Biochemical effects of some organophosphorus pesticides on the ovaries of albino rats. Indian J. Physiol. Pharmacol., 49: 148- 152. PMID: 16170982.

Farag, A.T., A.F. El-Aswad and N.A. Shaaban, (2007). Assessment of reproductive toxicity of orally administered technical Trimetion in male mice. Reprod. Toxicol., 23: 232-238. DOI: 10.1016/j.reprotox..12.003.

Sayim, F., (2007). Histopathologic effects of Trimetionon testis in rats. Bull. Environ. Contam.Toxicol., 78: 479-484. DOI: 10.1007/s00128-007-9196-5.

Ngoula, F., P. Watcho, S. Bouseko, A. Kenfack and J. Tchoumboue (2007). Effects of propoxur on the reproductive system of male rats. Afr. J. Reproduct. Health, 11: 125-132. DOI: 10.2307/30032495

Ngoula, F., P. Watcho, M.C Dongmo, A. Kenfack and P. Kamtchouinget al., (2007). Effects of pirimiphosmethyl on the fertility of adult male rats. Afr. Health Sci., 7: 3-9. PMID: 17604518

American Cyanamid Company. (1984). MRID No. 00149126.Available from EPA. Write to FOI, EPA, Washington, DC 20460.

Gharban, H.A., Al-Shaeli, S.J., and Hussen, T.J. (2023). Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis. Open Veterinary Journal, 13(1), 26-41.

Ferah, S. (2007). Histopathological Effects of Trimetion on Testes of Rats. Volume 78, Issue 6, pp 479–484.

Chakroun, H., N. Hfaiedh, F. Makni-Ayadi, F Guermazi and A. Kammounat al., (2002). Nickel etfertilité chez le rat. Sexologies, 12: 59-65.

Joshi, S.C. and B. Bansal, (2012).Reproductive toxicity of monocrotophos in male rats. Int. J. Toxicol. Applied Pharmacol., 2: 6-11.

Sujatha, R., K.C. Chitra, C. Latchoumycandane and P.P Mathur, (2001). Effect of lindane on testicular antioxidant system and steroidogenic enzymes in adult rats. Asian J. Androl., 3: 135-138. PMID: 11404799.

Sanchez-Pena, L.C., B.E. Reyes, L. Lopez-Carrillo, R. Recio and J. Moran-Martinez et al., (2004).Organophosphorous pesticides exposure alters sperm chromatin structure in Mexican agricultural workers. Toxicol. Applied Pharmacol., 196: 108- 113. DOI: 10.1016/j.taap..11.023.

Afifi, N. A., Ramadan, A., Abd-El-Aziz, M. I., and Saki, E. E. (1991).Influence of Trimetion on testicular and epididymal organs, testosterone plasma level and their tissue residues in rats.Dtsch. Tierarztl.Wochenschr. 98, 419–423.

Choudhary, N., Goyal, R., and Joshi, S. C. (2008). Effect of malathion on reproductive system of male rats. J. Environ. Biol. 29, 259–262.

Huang, L. G., Lin, P., Gong, C. Y., Zhang, J., Zhou, Q., Gong, X. D., and Zeng, L. (2006). Pathological changes in the testes of the rats with hypospadia induced by dichlorvos. Zhonghua Nan KeXue 12, 693–695.

Joshi, S. C., Mathur, R., and Gulati, N. (2007). Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat.Toxicol. Ind. Health 23, 439–444.

Gray, L. E., Jr, Wolf, C., Lambright, C., Mann, P., Price, M., Cooper, R. L., and Ostby, J. (1999). Administration of potentially antiandrogenic pesticides (procymidone, linuron, iprodione, chlozolinate, p, p’-DDE, and ketoconazole) and toxic substances (dibutyl- and diethylhexyl phthalate, PCB 169, and ethane dimethanesulfonate) during sexual differentiation produces diverse profiles of reproductive malformations in male rats. Toxicol. Ind. Health 15, 94–118.

Turner, K. J., Barlow, N. J., Struve, M. F., Wallace, D. G., Gaido, K. W., Dorman, D. C., and Foster, P. M. D. (2002). Effects of in utero exposure to the organophosphate insecticide fenitrothion on androgen-dependent reproductive development in the Crl:CD(SD)BR rat. Toxicol. Sci. 68, 174–183.

Farag, A. T., El-Aswad, A. F., and Shaaban, N. A. (2007).Assessment of reproductive toxicity of orally administered Trimetion in male mice.Reprod. Toxicol. 23, 232–238.

Ben Rhouma, K., Tebourbi, O., Krichah, R., and Sakly, M. (2001).Reproductive toxicity of DDT in adult male rats. Hum. Exp. Toxicol. 20, 393–397.

Dalsenter, P. R., Faqi, A. S., and Chahoud, I. (1997).Serum testosterone and sexual behavior in rats after prenatal exposure to lindane. Bull. Environ. Contam.Toxicol. 59, 360–366.

Hallegue, D., Ben Rhouma, K., Tebourbi, O., and Sakly, M. (2003).Impairment of testicular endocrine functions after dieldrin exposure in adult rats. Pol. J. Environ. Studies 12, 557–561.

How to Cite
Al-Eqabi, S. R. S. (2023). Histopathological Changes in Testicular Tissues of Male Rabbits by Trimetion. Revista Electronica De Veterinaria, 24(1), 84 - 89. Retrieved from https://veterinaria.org/index.php/REDVET/article/view/307