Probiotic Fermented Bitter Melon Juice as a Potential Adjuvant Therapy for Type 2 Diabetes: Evidence from Animal Model Research

  • Nayana Borah Assistant Professor, Department of Life Science, School of Sciences, JAIN (Deemed-to-be University), Karnataka, India
  • Jayabhaye Mahesh Shrirnag Professor, Department of Ayurveda, Sanskriti University, Mathura, Uttar Pradesh, India
  • Phool Chandra Professor, College of Pharmacy, Teerthanker Mahaveer University, Moradabad, Uttar Pradesh, India
Keywords: Enzyme, probiotics, acarbose, Fermentation, Diabetesl


Charantin, an antioxidant with anti-diabetic properties that act as a -glucosidase inhibitor, is found in the bitter melon Mordica charantia (MC). Lactic acid's antioxidant capabilities were enhanced by fermentation using Lactobacillus fermentum. The intention of the study was to compare various treatments to acarbose and examine how each affects blood sugar levels and Superoxide dismutase (SOD) levels before and after therapy—a technique for experimentation. A total of 28 male Sprague-Dawley rats were utilized. Streptozotocin (STZ) at a dose of 60 mg/kg was combined with nicotinamide at a dose of 120 mg/kg to cause type 2 diabetes. The animals were randomly randomized into four groups after STZ induction for three days. For a total of 28 days, acarbose (45 mg/100 feed), MC (15 ml/kg BW), and fermented MC (10 ml/ kg BW) were administered. The paired t-test was used to analyze how the data from the pretest and posttest differed. Glucose and Superoxide dismutase SOD concentrations were determined by spectrophotometer and enzyme-linked immunoassay (ELISA), respectively. Fixed at 0.05 was the threshold of significance results. Blood sugar levels in the MC and fermented MC group was significantly lower than those in the acarbose group during fasting and after meals. SOD values considerably rose in the groups that received MC and fermented MC but not to the same extent as in those that received acarbose. Acarbose and fermented MC have advantages, but MC significantly increased SOD and decreased fasting and postprandial blood glucose levels.


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How to Cite
Nayana Borah, Jayabhaye Mahesh Shrirnag, & Phool Chandra. (2023). Probiotic Fermented Bitter Melon Juice as a Potential Adjuvant Therapy for Type 2 Diabetes: Evidence from Animal Model Research. Revista Electronica De Veterinaria, 24(2), 222 - 232. Retrieved from