Using Commercial Cryopreservation of Dromedary Camel Semen Using Tris-Based Extenders (T-BE), Free-From and Enriched With Egg Yolks

  • Seydur Rahman Assistant Professor, Department of Zoology, Assam down Town University, Guwahati, Assam, India
  • Bansal RK Director, School of Agricultural Sciences, Jaipur National University, Jaipur, Rajasthan, India
  • Manashree Avinash Mane Assistant Professor, Department of Forensic Science, School of Sciences, JAIN (Deemed-to-be University), Karnataka, Bangalore, India
Keywords: Triladyl, Steridyl, AndroMed, OPTIXcell, SHOTOR, and Semen


Using commercial Tris-based extenders, the effectiveness of cryopreserving Semen from seven adult dromedary camels using both eggs yolk-supplemented and egg yolk-free methods were compared in this study. Control was provided via the SHOTOR camel-specific extension. AndroMed, OPTIXcell, Triladyl, Steridyl, SHOTOR, and Triladyl were employed to assess and dilute the collected semen samples. Before liquid nitrogen freezing, the dilute Two hours were spent progressively cooling and balancing the Semen. Using computer-assisted sperm analysis, the DNA fragmentation, anomalies, plasma membrane integrity, kinetics, vitality, and motility of the Semen were assessed both before and following freeze-thaw cycles. Progressively active sperm during the pre-freezing examination was greater in samples diluted with SHOTOR in data diluted with Steridyl, OPTIXcell, or AndroMed.Additionally, SHOTOR and Triladyl produced sperm with much higher viability and integrity of DNA than any other diluent, whereas Triladyl had sperm with significantly higher plasma membrane integrity. Triladyl significantly increased sperm motility in the post-thawing examination as opposed to SHOTOR, Steridyl, or AndroMed, with OPTIXcell as the lowest effective. The use of Triladyl produced the highest level of progressive sperm motility. AndroMed and Triladyl created the highest sperm velocities in curved, straight, and average routes after thawing, respectively. Triladyl generated sperm with the highest coefficients of linearity and straightness, while SHOTOR produced sperm with the greatest DNA and cell membrane integrity. Steridyl was less effective than Triladyl, while OPTIXcell and AndroMed led to subpar sperm quality after freezing viability. With OPTIXcell, the rate of aberrant sperm was highest.In contrast, SHOTOR or Triladyl had the lowest rate—however, the greatest sperm quality after Triladyl, SHOTOR, and semen cryopreservation provided thawing. Steridyl, AndroMed, and OPTIXcell are further viable treatments. Our research shows that Triladyl is currently the best commercially available extender for cryopreserving dromedary camel’s Semen.


Zuidema, D., Kerns, K. and Sutovsky, P., 2021. An Exploration of Current and Perspective Semen Analysis and Sperm Selection for Livestock Artificial Insemination. Animals 2021, 11, 3563.

Sathe, S., 2021. Cryopreservation of Semen. Bovine reproduction, pp.986-999.

Shahin, M.A., Khalil, W.A., Saadeldin, I.M., Swelum, A.A. and El-Harairy, M.A., 2021. Effects of vitamin C, vitamin E, selenium, zinc, or their nanoparticles on camel epididymal spermatozoa stored at 4 C. Tropical Animal Health and Production, 53, pp.1-9.

Swelum, A.A., Ba-Awadh, H.A., Olarinre, I.O., Saadeldin, I.M. and Alowaimer, A.N., 2022. Effects of adding mixed chicken and quail egg yolks to the cryo diluent on the quality of ram semen before and after cryopreservation. Frontiers in Veterinary Science, 9, p.1013533.

Miguel-Jimenez, S., Del Alamo, M.M.R., Alvarez-Rodriguez, M., Hidalgo, C.O., Peña, A.I., Muino, R., Rodríguez-Gil, J.E. and Mogas, T., 2020. In vitro assessment of egg yolk-, soya bean lecithin-and liposome-based extenders for cryopreservation of dairy bull semen. Animal Reproduction Science, 215, p.106315.

Al-Bulushi, S., Manjunatha, B.M., Bathgate, R., Rickard, J.P. and de Graaf, S.P., 2019. Liquid storage of dromedary camel semen in different extenders. Animal reproduction science, 207, pp.95-106.

Shahin, M.A., Khalil, W.A., Saadeldin, I.M., Swelum, A.A.A. and El-Harairy, M.A., 2020. Comparison between the effects of adding vitamins, trace elements, and nanoparticles to shot or extender on the cryopreservation of dromedary camel epididymal spermatozoa. Animals, 10(1), p.78.

Purohit, G.N., Vyas, S., Yadav, V., Nain, S., Chaudhary, A.K., Kumar, A., Dholpuria, S. and Saraswat, C.S., 2023. Semen characteristics and artificial insemination in dromedary camels. Small Ruminant Research, p.106911.

Malo, C., Elwing, B., Soederstroem, L., Lundeheim, N., Morrell, J.M. and Skidmore, J.A., 2019. Effect of different freezing rates and thawing temperatures on cryo survival of dromedary camel spermatozoa. Theriogenology, 125, pp.43-48.

Malo, C., Grundin, J., Morrell, J.M. and Skidmore, J.A., 2019. Individual male dependent improvement in post-thaw dromedary camel sperm quality after addition of catalase. Animal reproduction science, 209, p.106168.

Malo, C., Crichton, E.G., and Skidmore, J.A., 2020. Preservation of the spermatozoa of the dromedary camel (Camelus dromedarius) by chilling and freezing: the effects of cooling time, extender composition and catalase supplementation. Theriogenology, 153, pp.9-18.

O'Brien, E., Malo, C., Castaño, C., García-Casado, P., Toledano-Díaz, A., Martínez-Madrid, B., Rodriguez-Martinez, H., Álvarez-Rodríguez, M. and Santiago-Moreno, J., 2022. Sperm freezability is neither associated with the expression of aquaporin 3 nor sperm head dimensions in dromedary camel (Camelus dromedarius). Theriogenology, 189, pp.230-236.

Malo, C., De Blas, I., and Skidmore, J.A., 2021. Interaction among extenders, cryoprotectants, and Orvus Es Paste supplementation and freezing rate for sperm cryopreservation in the dromedary camel. Andrologia, 53(11), p.e14224.

Desantis, S., Lacalandra, G.M., Batista, M., Amann, O., Antonelli, D. and Monaco, D., 2021. Seminal plasma alters surface GLC profile of dromedary camel cryopreserved epididymal spermatozoa. Theriogenology, 167, pp.77-84.

Herrid, M., Nagy, P., Juhasz, J., Morrell, J.M., Billah, M., Khazanehdari, K. and Skidmore, J.A., 2019. Donor sperm production in heterologous recipients by testis germ cell transplantation in the dromedary camel. Reproduction, Fertility and Development, 31(3), pp.538-546.

How to Cite
Seydur Rahman, Bansal RK, & Manashree Avinash Mane. (2023). Using Commercial Cryopreservation of Dromedary Camel Semen Using Tris-Based Extenders (T-BE), Free-From and Enriched With Egg Yolks. Revista Electronica De Veterinaria, 24(2), 256 - 267. Retrieved from