Maximizing Broiler Chicken Production through In Ovo Injection of Vitamin E: Effects on Performance and Immune Function

  • Minakshi Bhattacharjee Associate Professor, Department of Biotechnology, Assam down Town University, Guwahati, Assam, India
  • Meena RL Assistant Professor, School of Agricultural Sciences, Jaipur National University, Jaipur, Rajasthan, India
  • Krupa S Assistant Professor, Department of Chemistry & Biochemistry, School of Sciences, JAIN (Deemed-to-be University), Karnataka, India
Keywords: Broiler chicken, Ovo injection, L-ascorbic acid (LA), hatching


Investigations were done into how different L-ascorbic acid (LA) injection levels affected the embryos' capacity to hatch and how much LA remained in their serum after the injection. Four different treatment groups were created at random from a total of 980 Ross 708 broiler eggs: non-injected saltwater without LA, saline (SL) injected with LA, and SL carrying either 12 or 27 mg of LA. The eggs were given a 100 μL amount of sterile SL (0.90%), either by itself or when combined with a single of the dual LA levels, during the 18-day implantation (doi) stage. Also calculated were the amount of egg weight reduction from doi 0 to doi 12 to doi. To ascertain the stage of embryo mortality, a hatch residue study was carried out after candling. The hatchability of living embryonic eggs (HI) and the weight (BW) of the hatchlings were calculated at around 21 doi. Serum LA levels were measured using blood samples collected 6, and 24 hours after LA injection. No treatment group differed from the others in terms of serum LA concentrations, HI, or hatchling BW. In contrast to the groups receiving 12 mg of LA in SL and SL alone, the non-injected group of chicks exhibited a greater (p = 0.05) at hatching fatality of embryos. These findings imply that high doses of LA (12 and 25 mg) administered in ovo have a chance to enhance embryonic viability without having a deleterious impact on HI or serum LA contents. The prolonged duration of LA in the cornea of the eye and other relevant tissues when subjected to various amounts of supplementary LA requires more investigation.



Mousstaaid, A., Fatemi, S.A., Elliott, K.E., Alqhtani, A.H. and Peebles, E.D., 2022. Effects of the in ovo injection of L-ascorbic acid on broiler hatching performance. Animals, 12(8), p.1020.

Edgar, J.A., 2019. L-ascorbic acid and the evolution of multicellular eukaryotes. Journal of Theoretical Biology, 476, pp.62-73.

Sobolev, O.I., Lisohurska, D.V., Pyvovar, P.V., Topolnytskyi, P.Р., Gutyj, B.V., Sobolievа, S.V., Borshch, О.O., Liskovich, V.А., Verkholiuk, M.M., Petryszak, O.Y. and Kuliaba, O.V., 2021. Modeling the effect of different dose of selenium additives in compound feed on the efficiency of broiler chicken growth. Ukrainian Journal of Ecology, 11(2), pp.292-299. F

Paz, I.C.D.L.A., de Lima Almeida, I.C., de La Vega, L.T., Milbradt, E.L., Borges, M.R., Chaves, G.H.C., dos Ouros, C.C., da Silva, M.I.L., Caldara, F.R. and Andreatti Filho, R.L., 2019. Productivity and well-being of broiler chickens supplemented with probiotic. Journal of Applied Poultry Research, 28(4), pp.930-942.

Humam, A.M., Loh, T.C., Foo, H.L., Izuddin, W.I., Awad, E.A., Idrus, Z., Samsudin, A.A. and Mustapha, N.M., 2020. Dietary supplementation of postbiotics mitigates adverse impacts of heat stress on antioxidant enzyme activity, total antioxidant, lipid peroxidation, physiological stress indicators, lipid profile and meat quality in broilers. Animals, 10(6), p.982.

Abou-Elkhair, R., Ahmed, H., Ketkat, S. and Selim, S., 2020. Supplementation of a low-protein diet with tryptophan, threonine, and valine and its impact on growth performance, blood biochemical constituents, immune parameters, and carcass traits in broiler chickens. Veterinary World, 13(6), p.1234.

Hassan, H.A., Arafat, A.R., Farroh, K.Y., Bahnas, M.S., El-Wardany, I. and Elnesr, S.S., 2022. Effect of in ovo copper injection on body weight, immune response, blood biochemistry and carcass traits of broiler chicks at 35 days of age. Animal Biotechnology, 33(6), pp.1134-1141.

Gao, T., Zhao, M.M., Zhang, L., Li, J.L., Yu, L.L., Lv, P.A., Gao, F. and Zhou, G.H., 2017. Effects of in ovo feeding of l-arginine on the development of lymphoid organs and small intestinal immune barrier function in posthatch broilers. Animal Feed Science and Technology, 225, pp.8-19.

Castañeda, C.D., McDaniel, C.D., Abdelhamed, H., Karsi, A. and Kiess, A.S., 2019. Evaluating bacterial colonization of a developing broiler embryo after in ovo injection with a bioluminescent bacteria. Poultry science, 98(7), pp.2997-3006.

Duan, A.Y., Ju, A.Q., Zhang, Y.N., Qin, Y.J., Xue, L.G., Ma, X., Luan, W.M. and Yang, S.B., 2021. The effects of in ovo injection of synbiotics on the early growth performance and intestinal health of chicks. Frontiers in Veterinary Science, 8, p.658301.

Zhang, H., Elliott, K.E.C., Durojaye, O.A., Fatemi, S.A. and Peebles, E.D., 2018. Effects of in ovo administration of L-ascorbic acid on broiler hatchability and its influence on the effects of pre-placement holding time on broiler quality characteristics. Poultry science, 97(6), pp.1941-1947.

Xu, X., Jackson, A.R. and Gonzalez, J.M., 2021. The effects of in ovo nicotinamide riboside dose on broiler myogenesis. Poultry Science, 100(3), p.100926.

Alcocer, H.M., Xu, X., Gravely, M.E. and Gonzalez, J.M., 2021. In Ovo Feeding of Commercial Broiler Eggs: An Accurate and Reproducible Method to Affect Muscle Development and Growth. JoVE (Journal of Visualized Experiments), (175), p.e63006.

Nouri, S., Ghalehkandi, J.G., Hassanpour, S. and Aghdam-Shahryar, H., 2018. Effect of in ovo feeding of folic acid on subsequent growth performance and blood constituents levels in broilers. International Journal of Peptide Research and Therapeutics, 24, pp.463-470.

Muh Ridwan, B., Rahardja, D.P. and Yusuf, M., 2019. The Effect of L-Glutamine and Broiler Albumin Supplementation through In Ovo Feeding Technique to Hatchability, Hatching Weight and Native Chicken Performance. International Journal of Current Innovations in Advanced Research, pp.35-42.

How to Cite
Minakshi Bhattacharjee, Meena RL, & Krupa S. (2023). Maximizing Broiler Chicken Production through In Ovo Injection of Vitamin E: Effects on Performance and Immune Function. Revista Electronica De Veterinaria, 24(2), 296 - 304. Retrieved from