A Preliminary Study of Regenerative Treatments for Horse Orthopaedic Disease

  • Intekhab Alam, Nisha, R Raghavendra
Keywords: Horse, Osteoarthritis (OA), Osteochondritis Dissecans (OCD), Treatments, Disease.

Abstract

Horses with orthopedic problems, especially those with joint abnormalities and osteoarthritis (OA), face significant obstacles to their overall health and athletic ability. The majority of current equine orthopedic disease therapies are soothing, providing momentary respite without addressing the contributing causes. Innovative techniques that support long-term functional rehabilitation and tissue regeneration are required for horse orthopedic diseases. The aim of this research is to assess the efficiency of regenerative treatments, particularly autologous platelet concentrates (PC), in treating orthopedic conditions in horses. To determine how the medicine affects joint effusion, lameness and general joint function. The study included seven horses with osteochondritis dissecans (OCD) a disease and osteoarthritis (OA). An autologous PC was prepared using a reliable and cost-effective technique. The horses received PC intra articular injections every two weeks and prior to each injection, as well as for two months following the last infusion, clinical assessments, lameness scores and joint evaluations were performed. Lameness ratings (p=0.048) and joint effusion (p=0.00043) showed significant improvements with the regeneration treatment. The horses, particularly those with OA, maintained improved joint function for eight months post-treatment. The upgrades in lameness and joint effusion that have been observed suggest possible advantages in fostering tissue regeneration as well as reducing inflammation.

References

Wismann, E. S., Jacobsen, S., Thøfner, M., Ladefoged, S., Ekstrøm, C., & Lindegaard, C. (2022). Long‐term athletic performance in sport horses after desmotomy of the accessory ligament of the deep digital flexor tendon. Equine Veterinary Journal, 54(3), 495-501. Doi:10.1111/evj.13472.

Crawford, K. L., Finnane, A., Greer, R. M., Phillips, C. J., Woldeyohannes, S. M., Perkins, N. R., & Ahern, B. J. (2020). Appraising the welfare of Thoroughbred racehorses in training in Queensland, Australia: the incidence and type of musculoskeletal injuries vary between two-year-old and older Thoroughbred racehorses. Animals, 10(11), 2046. Doi:10.3390/ani10112046.

Singh, R. P., Javaid, M., Haleem, A., Vaishya, R., & Ali, S. (2020). Internet of Medical Things (IoMT) for orthopedic in COVID-19 pandemic: Roles, challenges, and applications. Journal of clinical orthopedics and trauma, 11(4), 713-717.Doi: 10.1016/j.jcot.2020.05.011.

Monterrubio, C., & Pérez, J. (2021). Horses in leisure events: a posthumanist exploration of commercial and cultural values. Journal of Policy Research in Tourism, Leisure and Events, 13(2), 147-171.https://doi.org/10.1080/19407963.2020.1749063.

Pérez Fraile, A., González-Cubero, E., Martínez-Flórez, S., Olivera, E. R., & Villar-Suárez, V. (2023). Regenerative Medicine Applied to Musculoskeletal Diseases in Equines: A Systematic Review. Veterinary Sciences, 10(12), 666. Doi:10.3390/vetsci10120666.

Marycz, K., Szłapka-Kosarzewska, J., Geburek, F., & Kornicka-Garbowska, K. (2019). Systemic administration of rejuvenated adipose-derived mesenchymal stem cells improves liver metabolism in equine metabolic syndrome (EMS), a new approach in veterinary regenerative medicine. Stem Cell Reviews and Reports, 15, 842-850. Doi:10.1007/s12015-019-09913-3.

Shojaee, A., & Parham, A. (2019). Strategies of tenogenic differentiation of equine stem cells for tendon repair: current status and challenges. Stem cell research & therapy, 10(1), 1-13.Doi:10.1186/s13287-019-1291-0.

Mach, N., Ruet, A., Clark, A., Bars-Cortina, D., Ramayo-Caldas, Y., Crisci, E., ... & Lansade, L. (2020). Priming for welfare: gut microbiota is associated with equitation conditions and behavior in horse athletes. Scientific reports, 10(1), 8311.Doi:10.1038/s41598-020-65444-9.

Gallo, N., Natali, M. L., Sannino, A., & Salvatore, L. (2020). An overview of the use of equine collagen as an emerging material for biomedical applications. Journal of Functional Biomaterials, 11(4), 79. Doi:10.3390/jfb11040079.

Bagge, J., MacLeod, J. N., & Berg, L. C. (2020). Cellular proliferation of equine bone marrow and adipose tissue-derived mesenchymal stem cells decline with increasing donor age—frontiers in Veterinary Science, 7, 602403. Doi:10.3389/fvets.2020.602403.

Cequier, A., Sanz, C., Rodellar, C., & Barrachina, L. (2021). The usefulness of mesenchymal stem cells beyond the musculoskeletal system in horses. Animals, 11(4), 931. Doi:10.3390/ani11040931.

Ribitsch, I., Oreff, G. L., & Jenner, F. (2021). Regenerative medicine for equine musculoskeletal diseases. Animals, 11(1), 234.Doi:10.3390/ani11010234.

Mocchi, M., Dotti, S., Del Bue, M., Villa, R., Bari, E., Perteghella, S., ... & Grolli, S. (2020). Veterinary regenerative medicine for musculoskeletal disorders: can mesenchymal stem/stromal cells and their secretome be the new frontier? Cells, 9(6), 1453.Doi:10.3390/cells9061453.

Al Naem, M., Bourebaba, L., Kucharczyk, K., Röcken, M., & Marycz, K. (2020). Therapeutic mesenchymal stromal stem cells: Isolation, characterization, and role in equine regenerative medicine and metabolic disorders. Stem cell reviews and reports, 16, 301-322. Doi: 10.1007/s12015-019-09932-0.

Kaneps, A. J. (2023). A one-health perspective: use of hemoderivative regenerative therapies in canine and equine patients. Journal of the American Veterinary Medical Association, 261(3), 301-308.Doi:10.2460/javma.22.12.0556.

Bernardino, P. N., Smith, W. A., Galuppo, L. D., Mur, P. E., & Cassano, J. M. (2022). Therapeutics prior to mesenchymal stromal cell therapy improves outcome in equine orthopedic injuries—American Journal of Veterinary Research, 83(10).Doi:10.2460/ajvr.22.04.0072.

Sıtkıcan, O. K. U. R., YANMAZ, L. E., BEDİR, A. G., ŞENOCAK, M. G., ERSOZ, U., ORHUN, Ö. T., & KOCAMAN, Y. (2023). The Effectiveness of Thermography in Determining Localization of Orthopedic Diseases in Horses. Van Veterinary Journal, 34(1), 51-54.Doi:10.36483/vanvetj.1217002.

Fortier, L. A., Goodrich, L. R., Ribitsch, I., Schnabel, L. V., Shepard, D. O., Van de Walle, G. R., ... & Whealands Smith, R. K. (2020). One health in regenerative medicine: report on the second Havemeyer symposium on regenerative medicine in horses. Regenerative Medicine, 15(6), 1775-1787. Doi:10.2217/rme-2019-0143.

Garbin, L. C., & Olver, C. S. (2020). Platelet-rich products and their application to osteoarthritis. Journal of Equine Veterinary Science, 86, 102820. Doi: 10.1016/j.jevs.2019.102820.

Magri, C., Schramme, M., Febre, M., Cauvin, E., Labadie, F., Saulnier, N., ... & Maddens, S. (2019). Comparison of efficacy and safety of single versus repeated intra-articular injection of allogeneic neonatal mesenchymal stem cells for the treatment of osteoarthritis of the metacarpophalangeal/metatarsophalangeal joint in horses: a clinical pilot study. PLoS One, 14(8), e0221317. Doi: 10.1371/journal.pone.0221317

Published
2024-01-01
How to Cite
Intekhab Alam, Nisha, R Raghavendra. (2024). A Preliminary Study of Regenerative Treatments for Horse Orthopaedic Disease. Revista Electronica De Veterinaria, 24(3), 181-190. Retrieved from https://veterinaria.org/index.php/REDVET/article/view/408
Section
Articles