Divergent Diagnostic Approaches in Investigating Brucellosis: Unveiling Clinical Signs and Infection Rates
Abstract
Brucellosis, a zoonotic infectious disease caused by bacteria of the Brucella species, remains a significant global public health concern, impacting both humans and animals. To arrive at a thorough comprehension of the topic, an extensive evaluation was conducted, which involved examining 60 carefully obtained serum specimens from various demographic groups. These groups comprised veterinary students, those in charge of animal care, para-veterinarians, licensed veterinary technicians and experts in the complex field of synthetic animal conception. The study sought to provide a nuanced view on the number of cases and possible factors of brucellosis among the veterinary healthcare industry in the designated geographic region by deliberately included such a varied array of individuals. The study examined sera sample for brucellosis using various methods, including the “serum agglutination test (SAT), Immunoglobulin G (IgG) and Import General Manifest (IGM) indirect ELISA, Rose Bengal plate test (RBPT) and polymerase chain reaction (PCR)”. Demographic information such as clinical symptoms, age, risk factors and sex was collected through a structured questionnaire. Intermittent fever (71.62%) was the most common clinical sign observed, along with discomfort throughout the body. The guidelines for early identification, therapy and sickness follow-up highlight the need of a high degree of suspicion, perception and a multimodal testing method.
References
Yagupsky, P., Morata, P., & Colmenero, J. D. (2019). Laboratory diagnosis of human brucellosis. Clinical microbiology reviews, 33(1), 10-1128. DOI: https://doi.org/10.1128/cmr.00073-1
Muñoz, P. M., Mick, V., Sacchini, L., Janowicz, A., de Miguel, M. J., Cherfa, M. A., ... & Garofolo, G. (2019). Phylogeography and epidemiology of Brucella suis biovar 2 in wildlife and domestic swine. Veterinary microbiology, 233, 68-77. DOI: https://doi.org/10.1016/j.vetmic.2019.04.025
Mishra, A. R., Rani, P., Krishankumar, R., Ravichandran, K. S., & Kar, S. (2021). An extended fuzzy decision-making framework using hesitant fuzzy sets for the drug selection to treat the mild symptoms of Coronavirus Disease 2019 (COVID-19). Applied soft computing, 103, 107155. DOI: https://doi.org/10.1016/j.asoc.2021.107155
Yadav, D. D., Singh, N., Sreedharanunni, S., Hira, J. K., Chhabra, S., Trehan, A., ... & Das, R. (2021). Extreme Genotype/Phenotype Heterogeneity of Double Heterozygous Sickle β-Thalassemia in a Family: Implications in Antenatal Diagnosis. Indian Journal of Hematology and Blood Transfusion, 37, 689-691. DOI: 10.1007/s12288-021-01412-1
Sridhar, S., Situ, J., Cai, J. P., Yip, C. C. Y., Wu, S., Zhang, A. J. X., ... & Yuen, K. Y. (2021). Multimodal investigation of rat hepatitis E virus antigenicity: Implications for infection, diagnostics, and vaccine efficacy. Journal of Hepatology, 74(6), 1315-1324. DOI: https://doi.org/10.1016/j.jhep.2020.12.028
Richardson, S., Hill, R. M., Kui, C., Lindsey, J. C., Grabovksa, Y., Keeling, C., ... & Clifford, S. C. (2022). Emergence and maintenance of actionable genetic drivers at medulloblastoma relapse. Neuro-oncology, 24(1), 153-165. DOI: https://doi.org/10.1093/neuonc/noab178
Hu, T. H., Rosli, N., Mohamad, D. S., Kadir, K. A., Ching, Z. H., Chai, Y. H., ... & Singh, B. (2021). A comparison of the clinical, laboratory and epidemiological features of two divergent subpopulations of Plasmodium knowlesi. Scientific reports, 11(1), 20117. DOI: https://doi.org/10.1038/s41598-021-99644-8
Forsea, A. M. (2020). Melanoma epidemiology and early detection in Europe: diversity and disparities. Dermatology practical & conceptual, 10(3). DOI: https://doi.org/10.5826%2Fdpc.1003a33
Saha, S. K., Kong, D., Thursky, K., & Mazza, D. (2021). Divergent and convergent attitudes and views of general practitioners and community pharmacists to collaboratively implement antimicrobial stewardship programs in Australia: a nationwide study. Antibiotics, 10(1), 47. DOI: https://doi.org/10.3390/antibiotics10010047
Abduljalil, J. M., & Abduljalil, B. M. (2020). Epidemiology, genome, and clinical features of the pandemic SARS-CoV-2: a recent view. New microbes and new infections, 35, 100672. DOI: https://doi.org/10.1016/j.nmni.2020.100672
Machhi, J., Herskovitz, J., Senan, A.M., Dutta, D., Nath, B., Oleynikov, M.D., Blomberg, W.R., Meigs, D.D., Hasan, M., Patel, M. and Kline, P., 2020. The natural history, pathobiology, and clinical manifestations of SARS-CoV-2 infections. Journal of Neuroimmune Pharmacology, 15, pp.359-386. DOI: https://doi.org/10.1007/s11481-020-09944-5
Namdari, S., Nicholson, T., Abboud, J., Lazarus, M., Ramsey, M. L., Williams, G., & Parvizi, J. (2019). Comparative study of cultures and next-generation sequencing in the diagnosis of shoulder prosthetic joint infections. Journal of shoulder and elbow surgery, 28(1), 1-8. DOI: https://doi.org/10.1016/j.jse.2018.08.048
Traykov, V., Bongiorni, M. G., Boriani, G., Burri, H., Costa, R., Dagres, N., ... & Blomström-Lundqvist, C. (2019). Clinical practice and implementation of guidelines for the prevention, diagnosis and management of cardiac implantable electronic device infections: results of a worldwide survey under the auspices of the European Heart Rhythm Association. EP Europace, 21(8), 1270-1279. DOI: https://doi.org/10.1093/europace/euz137
Ren, D., Ren, C., Yao, R., Zhang, L., Liang, X., Li, G., ... & Gui, S. (2021). The microbiological diagnostic performance of metagenomic next-generation sequencing in patients with sepsis. BMC Infectious Diseases, 21, 1-9. DOI: https://doi.org/10.1186/s12879-021-06934-7
Galocha, M., Pais, P., Cavalheiro, M., Pereira, D., Viana, R., & Teixeira, M. C. (2019). Divergent Approaches to Virulence in C. albicans and C. glabrata: Two Sides of the Same Coin. International journal of molecular sciences, 20(9), 2345. DOI: https://doi.org/10.3390/ijms20092345
Alonso, D. O., Iglesias, A., Coelho, R., Periolo, N., Bruno, A., Córdoba, M. T., ... & Martínez, V. P. (2019). Epidemiological description, case‐fatality rate, and trends of Hantavirus Pulmonary Syndrome: 9 years of surveillance in Argentina. Journal of Medical Virology, 91(7), 1173-1181. DOI: https://doi.org/10.1002/jmv.25446
Heisser, T., Cardoso, R., Guo, F., Moellers, T., Hoffmeister, M., & Brenner, H. (2021). Strongly divergent impact of adherence patterns on efficacy of colorectal cancer screening: the need to refine adherence statistics. Clinical and Translational Gastroenterology, 12(9). DOI: https://doi.org/10.14309%2Fctg.0000000000000399
Kumar, R., Smith, K. S., Deng, M., Terhune, C., Robinson, G. W., Orr, B. A., ... & Northcott, P. A. (2021). Clinical outcomes and patient-matched molecular composition of relapsed medulloblastoma. Journal of Clinical Oncology, 39(7), 807. DOI: https://doi.org/10.1200%2FJCO.20.01359
Holtgrave, D. R., Barranco, M. A., Tesoriero, J. M., Blog, D. S., & Rosenberg, E. S. (2020). Assessing racial and ethnic disparities using a COVID-19 outcomes continuum for New York State. Annals of epidemiology, 48, 9-14. DOI: https://doi.org/10.1016/j.annepidem.2020.06.010
He, Y., Wang, J., Li, F., & Shi, Y. (2020). Main clinical features of COVID-19 and potential prognostic and therapeutic value of the microbiota in SARS-CoV-2 infections. Frontiers in Microbiology, 11, 1302. DOI: 10.1016/j.immuni.2012.04.011
Koyuncu, I., Kocyigit, A., Ozer, A., Selek, S., Kirmit, A., & Karsen, H. (2018). Diagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis. Central European Journal of Immunology, 43(1), 81-89. DOI: https://doi.org/10.5114/ceji.2018.74877
