Comparative Seroprevalence Analysis of Paratuberculosis in Goats and Cattle: Insights and Implications
Abstract
A chronic illness that affects cattle and other ruminants is called paratuberculosis (PTB). Severe diarrhea and weight loss are its defining characteristics. Eventually, after a protracted illness, the afflicted animals pass away. The primary site of infection for Mycobacterium avium subspecies paratuberculosis (MAP) is the intestines, where it causes intestinal wall thickening and chronic inflammation. This study's goal was to compare the seroprevalence of PTB in goats and cattle to determine the prevalence of MAP antibodies in these two different species. In six districts of Rajasthan, namely, Dausa, Bikaner, Jaisalmer, Jaipur, Udaipur and Dholpur an overall of 1951 blood specimens were taken from livestock holdings that appeared to be healthy, consisting of cattle and goats. Following the processing of the blood samples to separate the serum, an enzyme-linked immune sorbent test (ELISA) was performed to determine whether the samples had any antibodies against the infection. Only blood samples from the districts of Dausa and Bikaner were confirmed to be positive. It was discovered that the samples from the remaining districts were negative. The results of this study advance the understanding of the epidemiology of goat and cattle PTB, guiding the development of focused control measures for this disease that affects ruminant populations and has a substantial economic impact. However, this study suggests that the major areas of Rajasthan have low sickness prevalence.
References
Elsohaby, I., Fayez, M., Alkafafy, M., Refaat, M., Al-Marri, T., Alaql, F. A., ...& Elmoslemany, A. (2021). Serological and molecular characterization of Mycobacterium avium subsp. paratuberculosis (MAP) from sheep, goats, cattle and camels in the Eastern Province, Saudi Arabia. Animals, 11(2), 323. https://doi.org/10.3390/ani11020323
Rasmussen, P., Barkema, H. W., Mason, S., Beaulieu, E., & Hall, D. C. (2021). Economic losses due to Johne's disease (paratuberculosis) in dairy cattle. Journal of dairy science, 104(3), 3123-3143. https://doi.org/10.3168/jds.2020-19381
Idris, S. M., Eltom, K. H., Okuni, J. B., Ojok, L., Elmagzoub, W. A., El Wahed, A. A., ... & Gameel, A. A. (2021). Paratuberculosis: The hidden killer of small ruminants. Animals, 12(1), 12. https://doi.org/10.3390/ani12010012
Stabel, J. R., Bannantine, J. P., & Hostetter, J. M. (2020). Comparison of Sheep, Goats, and Calves as Infection Models for Mycobacterium avium subsp. paratuberculosis. Veterinary Immunology and Immunopathology, 225, 110060. https://doi.org/10.1016/j.vetimm.2020.110060
PourmahdiBorujeni, M., Haji Hajikolaei, M. R., Ghorbanpoor, M., Elhaei Sahar, H., Bagheri, S., & Roveyshedzadeh, S. (2021). Comparison of Mycobacterium avium subsp. paratuberculosis infection in cattle, sheep and goats in the Khuzestan Province of Iran: Results of a preliminary survey. Veterinary Medicine and Science, 7(5), 1970-1979. https://doi.org/10.1002/vms3.559
Sanchez, M. P., Tribout, T., Fritz, S., Guatteo, R., Fourichon, C., Schibler, L., ...& Boichard, D. (2022). New insights into the genetic resistance to paratuberculosis in Holstein cattle via single-step genomic evaluation. Genetics Selection Evolution, 54(1), 67. https://doi.org/10.1186/s12711-022-00757-z
Aquilani, C., Confessore, A., Bozzi, R., Sirtori, F., & Pugliese, C. (2022). Precision Livestock Farming technologies in pasture-based livestock systems. Animal, 16(1), 100429. https://doi.org/10.1016/j.animal.2021.100429
Bhat, A. M., Malik, H., Mir, M. S., Chaubey, K. K., & Singh, S. V. (2020). Cross-sectional study on seroprevalence and risk factor analysis of Mycobacterium avium subsp. paratuberculosis in Kashmir Merino sheep flocks of Central Kashmir valley, India. Small Ruminant Research, 193, 106266. https://doi.org/10.1016/j.smallrumres.2020.106266
Barrero‐Domínguez, B., Luque, I., Huerta, B., Gomez‐Laguna, J., Galán‐Relaño, Á., Gómez‐Gascón, L., ... & Astorga, R. J. (2019). Paratuberculosis in dairy goat flocks from southern Spain: risk factors associated with seroprevalence. Veterinary Record, 185(19), 600-600. https://doi.org/10.1136/vr.105465
Selim, A., Abdelhady, A., & Abdelrahman, A. (2021). Ovine Paratuberculosis: Seroprevalence and comparison of fecal culture and direct fecal PCR assay. Comparative Immunology, Microbiology and Infectious Diseases, 74, 101526. https://doi.org/10.1016/j.cimid.2020.101526
Selim, A., Ali, A. F., & Ramadan, E. (2019). Prevalence and molecular epidemiology of Johne’s disease in Egyptian cattle. Actatropica, 195, 1-5. https://doi.org/10.1016/j.actatropica.2019.04.019
Okuni, J. B., Hansen, S., Eltom, K. H., Eltayeb, E., Amanzada, A., Omega, J. A., ...& Ojok, L. (2020). Paratuberculosis: a potential zoonosis and a neglected disease in Africa. Microorganisms, 8(7), 1007. https://doi.org/10.3390/microorganisms8071007
Mohammed, T., Mamo, G., Zewude, A., Sirak, A., Gumi, B., & Ameni, G. (2023). Prevalence of paratuberculosis in cattle based on gross and microscopic lesions in Ethiopia. BMC Veterinary Research, 19(1), 203. https://doi.org/10.3390/microorganisms8071007
Caldow, G. L., Douglas, L., Thacker, J., Mackay, E., & Berezowski, J. (2023). Estimation of the specificity of an antibody ELISA for paratuberculosis generated from a sector of the UK cattle population using results from a paratuberculosis control programme. Preventive Veterinary Medicine, 215, 105910. https://doi.org/10.1016/j.prevetmed.2023.105910
Juste, R. A., Geijo, M. V., Elguezabal, N., Sevilla, I. A., Alonso-Hearn, M., & Garrido, J. M. (2021). Paratuberculosis vaccination specific and non-specific effects on cattle lifespan. Vaccine, 39(11), 1631-1641. https://doi.org/10.1016/j.vaccine.2021.01.058
Pickrodt, C., Köhler, H., Moog, U., Liebler-Tenorio, E. M., & Möbius, P. (2023). Molecular Diversity of Mycobacterium avium subsp. paratuberculosis in Four Dairy Goat Herds from Thuringia (Germany). Animals, 13(22), 3542. https://doi.org/10.3390/ani13223542
Fernández, M., Royo, M., Arteche-Villasol, N., Ferreras, M. C., Benavides, J., & Pérez, V. (2022). Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis. Vaccines, 10(10), 1709. https://doi.org/10.3390/vaccines10101709
Vass-Bognár, B., Bakony, M., Baumgartner, W., Khol, J. L., & Jurkovich, V. (2022). Association between calf rearing technology and farm-level paratuberculosis infection in Hungarian dairy farms. Preventive Veterinary Medicine, 207, 105719. https://doi.org/10.1016/j.prevetmed.2022.105719
Alonso-Hearn, M., Badia-Bringué, G., & Canive, M. (2022). Genome-wide association studies for the identification of cattle susceptible and resilient to paratuberculosis. Frontiers in Veterinary Science, 9, 935133. https://doi.org/10.3389/fvets.2022.935133
Ssekitoleko, J., Ojok, L., Omala, S. K., Mukhtar, M. E., Eltom, K. H., Eltayeb, E. S., ... & Okuni, J. B. (2022). Molecular and serological survey of paratuberculosis in cattle in selected districts of Western Uganda. BMC Veterinary Research, 18(1), 438. https://doi.org/10.1186/s12917-022-03535-7