Evaluate Activity of Chitosan Nanoparticles (CHNPS) as Antibacterial Against Some MDR Pathogenic Bacteria
Keywords:
Antibacterial activity, Chitosan Nanoparticles, MIC
Abstract
Chitosan nanoparticles (ChNPs) are recognized to show antibacterial action against a wide spectrum of diseases in this regard. As a result, they have emerged as a promising contender for combating multi-drug resistance.
Gram-positive and Gram-negative bacteria were obtained from infected burns and wounds. The antibacterial activity of ChNPs was performed using the disk diffusion method in various doses (500, 250, 125, 62.5, and 31.25 g/ml). Each isolate's MIC and MBC were also measured. ChNPs have shown dose-dependent antibacterial activity, and have even surpassed certain antibiotics in terms of efficiency. The MICs of ChNPs ranged from 125 to 62.5 g/ml, whereas the MBCs ranged from 250 to 500 g/ml. When GNPs were employed at the correct concentration, bacterial growth was significantly halted. ChNPs are recommended as a low-cost antibacterial option, particularly for treating ectopic infections without the danger of bacteria.
References
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[2] M. S. Usman, M. E. El Zowalaty, K. Shameli, N. Zainuddin, M. Salama, and N. A. Ibrahim, Int. J. Nanomed., 8, 4467 (2013).
[3] L. Didenko, D. Gerasimenko, N. Konstantinova, T. Silkina, I. Avdienko, G. Bannikova, and V. Varlamov, B Exp. Biol. Med., 140, 356 (2005).
[4] Toan NV, Ng Ch, Aye KN, et al. Production of high-quality chitin and chitosan from preconditioned shrimp shells. J Chem Technol Biotechnol. 2006;81(7):1113–1118.
[5] No hK, Park Ny, lee Sh, et al. Antibacterial activity of chitosans and chitosan oligomers with different molec- ular weights. Int J Food Microbiol. 2002;74(1-2):65–72.
[6] Devlieghere F, Vermeulen A, Debevere J. Chitosan: an- timicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiol. 2004;21(6):703–714.
[7] larsen Mu, Seward M, Tripathi A, et al. Biocompatible nanoparticles trigger rapid bacteria clustering. Biotechnol Prog. 2009;25(4):1094–1102.
[8] Azadi G, Seward M, larsen Mu, et al. Improved antimi- crobial potency through synergistic action of chitosan microparticles and low electric field. Appl Biochem Biotechnol. 2012;168(3):531–541.
[9] Mahamda, HA and Al Alwany AA, Influence of Syphilis Infection on Abortions in Iraq. Journal of Communicable Diseases, 2022. 54(4): 41-45
[10] Al Alwany AA, Effect and benefit of percutaneous coronary intervention in chronic total occlusion on ventricular repolarization: QT correction and dispersion, Journal of Medicine and Life, 2022, 15 (8),1025 - 1030, DOI: 10.25122/jml-2022-0207 Al Alwany AA, Echocardiographic Assessment of the Aortic Stenosis Valve Area: Parameters and Outcome. Journal of Medicinal and Chemical Sciences, 2022. 5(7): 1281-1288.
[11] Jaloud RE, Hassan FF, Al-Aamery RA, Hashim RT, Histopathological and Immunohistochemical Study of Aflatoxin B1 in Freshly Slaughtered Iraqi Sheep Meat, using CD Marker of TNF-α, International Journal of Drug Delivery Technology, 2022, 12 (2), 798 - 804,
[12] Kong M, Chen XG, Xing K, et al. Antimicrobial properties of chitosan and mode of action: a state of the art review. Int J Food Microbiol. 2010;144(1):51–63.
[13] hafdani FN, Sadeghinia N. A review on application of chitosan as a natural antimicrobial. World Acad Scieng Technol. 2011; 50:252–256.
[14] Ahmed S, Ahmad M, Ikram S. Chitosan: a natural anti- microbial agent – a review. J Appl Chem. 2014;3:493–503.
[15] Xing K, Zhu X, Peng X, et al. Chitosan antimicrobial and eliciting properties for pest control in agriculture: a review. Agron Sustain Dev. 2015;35(2):569–588.
[16] Sahariah P, Masson M. Antimicrobial chitosan and chi- tosan derivatives: a review of the structure–activity relationship. Biomacromolecules. 2017;18(11):3846–3868.
[17] Abd el-hack Me, el-Saadony MT, Shafi Me, et al.Antimicrobial and antioxidant properties of chitosan and its derivatives and their applications: a review. Int J Biol Macromol. 2020;164:2726–2744.
[18] Zhang h, oh M, Allen C, et al. Monodisperse chitosan nanoparticles for mucosal drug delivery. Biomacromolecules. 2004;5(6):2461–2468.
[19] Kawashima y, handa T, Kasai A, et al. Novel method for the preparation of controlled-release theophylline gran- ules coated with a polyelectrolyte complex of sodium polyphosphate-chitosan. J Pharm Sci. 1985;74(3):264– 268.
[20] ohya y, Shiratani M, Kobayashi h, et al. Release behav- ior of 5-fluorouracil from chitosan-gel nanospheres immobilizing 5-fluorouracil coated with polysaccharides and their cell specific cytotoxicity. J Macromol Sci Pure Appl Chem. 1994;31(5):629–642.
[21] Wang Sl, hiep DM, luong PM, et al. Preparation of chitosan nanoparticles by spray drying, and their anti- bacterial activity. Res Chem Intermed. 2014;40:2165– 2175.
[22] Tokumitsu h, Ichikawa h, Fukumori y. Chitosan-gadopentetic acid complex nanoparticles for gadolinium neutron-capture therapy of cancer: prepa- ration by novel emulsion-droplet coalescence technique and characterization. Pharm Res. 1999;16(12):1830– 1835.
[23] Melo eP, Aires-Barros MR, Cabral JMS. Reverse micelles and protein biotechnology. Biotechnol Ann Rev. 2001;7:87–129.
[24] luque-Alcaraz AG, lizardi-Mendoza J, Goycoolea FM, et al. Preparation of chitosan nanoparticles by nano- precipitation and their ability as a drug nanocarrier. RSC Adv. 2016;6(64):59250–59256.
[25] Alonso MJ. Nanoparticulate drug carrier technology. Drugs Pharm. Sci. 1996;77:203–242.
[26] hu y, Jiang X, Ding y, et al. Synthesis and characteriza- tion of chitosan–poly (acrylic acid) nanoparticles. Biomaterials. 2002;23(15):3193–3201.
[27] Kong M, Chen XG, Xing K, Park HJ. Antimicrobial properties of chitosan and mode of action: a state of the art review. Int J Food Microbiol. 2010;144(1):51–63. doi:10.1016/j.ijfoodmicro.2010.09.012
[28] Rabea EI, Badawy ME, Stevens CV, Smagghe G, Steurbaut W. Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules. 2003;4(6):1457–1465. doi:10.1021/bm034130m
[29] Goy RC, de Britto DD, Assis OBG OBG. A review of the antimicrobial activity of chitosan. Ciência e Tecnologia. 2009;19(3):241–247.
[30] Abbas HH, Al-Alwany AA, and FS Dleikh FS, Impact of smoking on cardiac electrophysiological parameters of symptomatic sinus node patients in Iraq. Pakistan Journal of Medical and Health Sciences, 2020, 14(4): 1643-1650.
[31] Jaloud RE, Hassan F.F., Isolation and identification of fungal propagation in Iraqi meat and detection of aflatoxin B1 using ELISA technique, Journal of Global Pharma Technology, 2018, 10 (8), 231-240.
[32] Divya K, Vijayan S, George TK, Jisha MS. Antimicrobial properties of chitosan nanoparticles: mode of action and factors affecting activity. Fibers Polym. 2017;18(2):221–230.
[33] Wardani G, Mahmiah SSA. In vitro antibacterial activity of chitosan nanoparticles against Mycobacterium tuberculosis. Pharmacogn J. 2018;10(1):162–166.
[34] Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational, Supplement. CLSI Document M02-A10 and M07-A8. Texas: Clinical and Laboratory Standards Institute; 2021.
[35] Frey FM, Meyers R. Antibacterial activity of traditional medicial plants used by Haudenosaunee peoples of New York State. J Int Soc Complement Med Res 2010;10:64.
[36] Clinical and Laboratory Standards Institute.(2021).Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational, Supplement. CLSI Document M02-A10 and M07-A8. Texas: Clinical and Laboratory Standards Institute; 2021.
[37] Khan, F.; Manivasagan, P.; Lee, J. W.; Pham, D. T. N.; Oh, J. and Kim, Y. M. (2019). Biofilm Inhibition, Attenuation of Virulence and Motility Properties in Pseudomonas aeruginosa PAO1. Mar. Drugs 17(3), 208-227.
[38] Haldorai, Y. & Shim, J.-J. Multifunctional chitosan–copper oxide hybrid material: photocatalytic and antibacterial activities. Int. J. Photoenergy 2013 (2013).
[39] Ahmad R, Sardar M. TiO2 nanoparticles as an antibacterial agents against E. coli. Int J Innov Res Sci Eng Technol 2013;2:2319-8753
[40] Abdulazeem, A.; AL-Amiedi, B.H.H.; Alrubaei, H.A. and Yasir H. AL-Mawlah. (2019). Titanium dioxide nanoparticles as antibacterial agents against some pathogenic bacteria. Drug Invention Today | Vol 12 • Issue 5 • 2019.pp:963-967.
Published
2023-12-31
How to Cite
Rabaa Mzahim Merza, et al. (2023). Evaluate Activity of Chitosan Nanoparticles (CHNPS) as Antibacterial Against Some MDR Pathogenic Bacteria. Revista Electronica De Veterinaria, 24(4), 287-292. Retrieved from https://veterinaria.org/index.php/REDVET/article/view/493
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