Genetic Comparison of Some Virulence Genes in Staphylococcus Aureus bacteria Isolated from Clinical Samples and Normal Flora
Abstract
A total of 100 samples were collected to investigate the bacteria of Staphylococcus aureus, distributed as follows (30 samples were collected from healthy people, 35 Wounds, and 35 burns were collected from patients admitted to Marjan Teaching Hospital.
It was found 40 isolates belonging to Staphylococcus aureus were distributed as follows( 21 based on phenotypic, culture, and biochemical characteristics.
The study showed that bacteria isolated from pathological conditions may produce extracellular enzymes that contribute to pathogenicity, including urease, hemolysin, catalase, and coagulase at 100%, while isolated from normal persons produced coagulase enzymes by 70% and 100% of the rest of the enzymes including catalase, hemolysin, and urease. A total of 20 isolates of S. aureus bacteria (pathogenic and normal flora) were selected depending on virulence factors produce to investigate some virulence genes.
A molecular study of some virulence genes of S. aureus, which are the coa, which encodes the production of the coagulase enzyme, and the hly, which encodes for the production of the hemolysin enzyme, indicated that all of the chosen sick isolates had the genes present, but just six of the normal flora isolates had the coa.
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