Synthesis and in vitro biological evaluation of metal complexes containing Pyridine

  • Minakshi Kumari
  • Mohd. Hussain
  • Aditay Kumar
  • Mamta khatak
  • Mohd. Isfaq
  • Mayank Mehendiratta
  • Mahesh kumar
Keywords: .


In the present study, metal complexes containing pyridine have been synthesized and characterized by physicochemical and spectral means. Broth microdilution (96 well microtiter plate) method was used to evaluate antimicrobial activity of the synthesized compounds against Gram positive bacteria (B. subtilis), Gram negative bacteria (E. coli) and fungal species (A.terrus & A.niger ) using cefadroxil (antibacterial) and fluconazole (antifungal) as standard drugs. Among the synthesized compounds, chalcone ligand C1 showed good antifungal activity. against A.terrus & A.niger (MIC =3.12µg/ml.) while complex KP1 showed significant activity against E. coli (MIC=1.56µg/ml) , A. niger (MIC=3.12µg./ml) and A. terrus (MIC=1.56µg/ml). Complex KP7 showed significant activity against B.subtilis and E.coli.(MIC = 3.12 µg/ml.). Antioxidant activity of the synthesized compounds was determined using D.P.P.H. assay method. Complex KP2 showed excellent antioxidant activity (48.46). The order of antioxidant activity showed the increase in antioxidant activity KP2>KP3> KP6>KP5>KP4>>KP7>C2>KP1>KP8>C1

Author Biographies

Minakshi Kumari

Department of Pharmaceutical Sciences, MDU Rohtak

Mohd. Hussain

Department of Pharmaceutical Sciences, MDU Rohtak

Aditay Kumar

Department of Pharmaceutical Sciences, MDU Rohtak

Mamta khatak

Assistant Professor, BM Group of Institution, Farukhnagar, Gurugram

Mohd. Isfaq

MVN University, Palwal

Mayank Mehendiratta

Department of Pharmaceutical Sciences, MDU Rohtak

Mahesh kumar

Associate Professor, Department of Pharmaceutical Sciences, MDU Rohtak



1. Bourichi S, Misbahi H, Rodi YK, Chahdi fo, szabó S, szalontai B, gajdács M, molnár J, spengler G. In vitro evaluation of the multidrug resistance reversing activity of novel imidazo [4, 5-b] pyridine derivatives. Anticancer Research. 2018 Jul 1;38(7):3999-4003.
2. Sies H. Oxidative stress: a concept in redox biology and medicine. Redox biology. 2015 Apr 1;4:180-3.
3. Marinescu M, Popa CV. Pyridine Compounds with Antimicrobial and Antiviral Activities. International Journal of Molecular Sciences. 2022 Jan;23(10):5659.
4. Khan E. Pyridine derivatives as biologically active precursors; organics and selected coordination complexes. Chemistry Select. 2021 Apr 8;6(13):3041-64.
5. Nagashree S, Karthik CS, Sudarshan BL, Mallesha L, Spoorthy HP, Sanjay KR, Mallu P. In vitro antimicrobial activity of new 2-amino-4-chloropyridine derivatives: A structure-activity relationship study. Journal of Pharmacy Research. 2015 Aug;9(8):509-16.
6. Zhuang C, Zhang W, Sheng C, Zhang W, Xing C, Miao Z. Chalcone: a privileged structure in medicinal chemistry. Chemical reviews. 2017 Jun 28;117(12):7762- 810.
7. Johnson J, Yardily A. Co (II), Ni (II), Cu (II), and Zn (II) metal complexes of chalcone: Synthesis, characterization, thermal, antimicrobial, photocatalytic degradation of dye and molecular modeling studies. Applied Organometallic Chemistry. 2022 Jan;36(1):e6465.
8. Rammohan A, Reddy JS, Sravya G, Rao CN, Zyryanov GV. Chalcone synthesis, properties and medicinal applications: a review. Environmental Chemistry Letters. 2020 Mar;18(2):433-58.
9. Johnson J, Yardily A. Chalconoid metal chelates: spectral, biological and catalytic applications. Journal of Coordination Chemistry. 2019 Aug 3;72(15):2437-88.
10. Lamara KO, Makhloufi-Chebli M, Benazzouz-Touami A, Terrachet-Bouaziz S, Robert A, Machado-Rodrigues C, Behr JB. Synthesis, biological activities of chalcones and novel 4-acetylpyridine oximes, molecular docking of the synthesized products as acetylcholinesterase ligands. Journal of Molecular Structure. 2022 Mar 15;1252:132153.
11. Sulpizio C, Breibeck J, Rompel A. Recent progress in synthesis and characterization of metal chalcone complexes and their potential as bioactive agents. Coordination Chemistry Reviews. 2018 Nov 1;374:497-524.
12. Cappuccino JC, Sherman N (1999) Microbiology a laboratory manual. Addison Wesley, California, pp 6:263-265
13. Andrews JM. Determination of minimum inhibitory concentrations. Journal of antimicrobial Chemotherapy. 2001 Jul 1;48(suppl_1):5-16.
14. Tang Q, Hu J, Ding YJ, Zhang YF, Li HF, Xu M, Yang XC, Liang L, Li WG. Syntheses, structures and antimicrobial properties of complexes based on 2- hydroxybenzaldehyde-4-aminoantipyrine Schiff base. Journal of Coordination Chemistry. 2021 Mar 23;74(4-6):1039-52.
15. Lone IH, Khan KZ, Fozdar BI, Hussain F. Synthesis antimicrobial and antioxidant studies of new oximes of steroidal chalcones. Steroids. 2013 Sep 1;78(9):945- 50.
16. Mukherjee PK. Pharmacological screening of herbal drugs. Quality control of herbal drugs. 2012;564.
17. Singh R, Shushni MA, Belkheir A. Antibacterial and antioxidant activities of Mentha piperita L. Arabian Journal of Chemistry. 2015 May 1;8(3):322-8.
18. Chandrashekaraiah M, Lingappa M, Gowda V, Bhadregowda DG (2014) Synthesis of some new pyrimidine-azitidinone analogues and their antioxidant, in vitro antimicrobial and antitubercular activities. J chem 2014
19. Elewady GY (2008) Pyrimidine derivatives as corrosion inhibitors for carbon-steel in 2M hydrochloric acid solution. Int J Electrochem. Sci 3(10):1149
20. Gulcan M, Sonmez M, Berber I (2012) Synthesis, characterization, and antimicrobial activity of a new pyrimidine Schiff base and its Cu (II), Ni (II), Co (II), Pt (II), and Pd (II) complexes. Turkish Journal of Chemistry 36(1):189-200.
21. Latif K, Abdelall E, Abdelgawad M, Ahmed R, Bakr R (2014) Synthesis and anticancer activity of some new pyrazolo [3, 4-d] pyrimidin-4-one derivatives. Molecules 19(3):3297-3309
22. Martins P, Jesus J, Santos S, Raposo L, Roma-Rodrigues C, Baptista P, Fernandes A (2015) Heterocyclic anticancer compounds: recent advances and the paradigm shift towards the use of nanomedicine’s tool box. Molecules 20(9):16852-16891
23. Mohamed MM, Khalil AK, Abbass EM, Naggar AM (2017) Design, synthesis of new pyrimidine derivatives as anticancer and antimicrobial agents. Synthetic Communications 47(16):1441-1457
24. Mohamed MS, Awad M, Sayed AI (2010) Synthesis of certain pyrimidine derivatives as antimicrobial agents and anti-inflammatory agents. Molecules 15(3):1882- 1890
25. Sayed AA, Shahat M, Rabie ST, Flefel EM, Elshafyc DN (2015) New pyrimidine and fused pyrimidine derivatives: synthesis and anti-Hepatitis A virus (HAV) evaluation. Int J Pharm (5):69-79
26. Senbagam R, Rajarajan M, Vijayakumar R, Manikandan V, Balaji S, Vanangamudi G, Thirunarayana G (2015) Synthesis, spectral correlations and antimicrobial activities of 2-pyrimidine Schiff’s bases. Int Lett Chem Phy Astro (53):154-164
27. The Indian Pharmacopoeia Commission (2018) Pharmacopoeia of India, vol. Ӏ. Controller of publication, ministry of health department, Govt. of India, New Delhi, pp 37
How to Cite
Minakshi Kumari, Mohd. Hussain, Aditay Kumar, Mamta khatak, Mohd. Isfaq, Mayank Mehendiratta, & Mahesh kumar. (2024). Synthesis and in vitro biological evaluation of metal complexes containing Pyridine. Revista Electronica De Veterinaria, 25(1S), 207-219. Retrieved from