Extraction and Evaluation of Methanolic Extract of Arachis Hypogaea Leaves for Anti-Microbial Activity

  • Rajveer Singh
  • Shaily Mishra
  • Dr. Shamim Ahmad
  • Dr. Nasiruddin Ahmad Farooqui
Keywords: Extraction, plant-herbs, phytoconstituents, extract, Arachis Hypogaea (AH), anti-microbial activity, methanolic extraction, phyto-therapeutics.


Objective: This study aimed to extract and evaluate the antimicrobial properties of methanolic extract from the leaves of Arachis hypogaea AH against various pathogenic microorganisms.

Materials and Methodology: The leaves of AH, methanol and distilled water as solvent, Sterile discs and ethanol as sterilization purposes were purchased from Sigma-Aldrich, Institute, Fisher scientific and Sigma Aldrich respectively. AH collected, dried, and subjected to methanolic extraction using a Soxhlet apparatus. The resulting crude extract was concentrated and then tested for antimicrobial activity. The antimicrobial efficacy was assessed using the agar well diffusion method against a panel of E.coli, B.cereus and Bacillus subtilis as the tested microorganisms.

Results: The methanolic extract of AH leaves exhibited significant antimicrobial activity E.coli, B.cereus and Bacillus subtilis F6 was optimized formulation. The zones of inhibition observed ranged from 10 to 20 mm indicating a broad-spectrum antimicrobial effect. The interpretation of microorganisms E.coli, B.cereus and Bacillus subtilis shows moderate, good and excellent activity.

Conclusion: The methanolic extract of AH leaves possesses potent antimicrobial properties, which could be attributed to the presence of bioactive compounds in the leaves. These findings suggest that AH leaf extract could be a valuable source of natural antimicrobial agents for therapeutic applications.

Author Biographies

Rajveer Singh

Research scholar, Translam Institute of Pharmaceutical Education and Research Meerut, Uttar Pradesh, India.

Shaily Mishra

Associate professor, Translam Institute of Pharmaceutical Education and Research Meerut, Uttar Pradesh, India.

Dr. Shamim Ahmad

Director, Translam Institute of Pharmaceutical Education and Research Meerut, Uttar Pradesh, India.

Dr. Nasiruddin Ahmad Farooqui

Professor & HOD, Translam Institute of Pharmaceutical Education and Research Meerut, Uttar Pradesh, India.


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How to Cite
Rajveer Singh, Shaily Mishra, Dr. Shamim Ahmad, & Dr. Nasiruddin Ahmad Farooqui. (2024). Extraction and Evaluation of Methanolic Extract of Arachis Hypogaea Leaves for Anti-Microbial Activity. Revista Electronica De Veterinaria, 25(1S), 267-275. https://doi.org/10.53555/redvet.v25i1S.621