In Vivo, In Vitro Rt-Pcr Based Gene Expression Studies On Biological Activities Of Biosynthesized Silver Nanoparticles From Tinospora Cordifolia (Thunb.) Plant Extract Against Vibriosis Infection In The Shrimp Litopenaeus vannamei (Boone, 1931)
Keywords:
Antibacterial activity, RT-PCR, SEM, silver nanoparticles, XRD, shrimp
Abstract
MDR or multi-drug resistance resulting from the treatment of diseases in shrimp culture is one of the primary difficulties confronting the shrimp business. Shrimp are one of the most popular seafood commodities sold globally worldwide, with aquaculture accounting for the bulk of output. While there are a number of hazardous microbes known to exist in shrimp farming, vibrio strains in particular are a major cause of acute hepatopancreatic necrosis disease (AHPND), which causes severe production losses in the widely farmed white leg shrimp (Litopenaeus vannamei). Antimicrobial resistance is spreading across the globe, making disease control critical. It has been shown that medicinal plants have high concentrations of antimicrobial compounds. This work assessed the antibacterial and antioxidant properties of silver nanoparticles synthesized from a plant extract called Tinospora cordifolia. An investigation on RT-PCR gene expression was conducted. The purpose of the study is to assess the antibacterial properties of plant Tinospora cordifolia against vibriosis infection in shrimp.
References
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27. Sørum, H. (1999). Antibiotic resistance in Aquaculture. Acta Veterinaria Scandinavia, Supplementum, 92, 29–36.
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29. Tacon, A. G. (2020). Trends in global aquaculture and aquafeed production: 2000–2017. Reviews in Fisheries Science & Aquaculture, 28(1), 43-56.
30. Teng, T., Liang, L., Chen, K., Xi, B., Xie, J. and Xu, P. (2017). Isolation, identification and phenotypic and molecular characterization of pathogenic Vibrio vulnificus isolated from Litopenaeus vannamei. PLoS One, 12(10), e0186135.
31. Thornber, K., Verner‐Jeffreys, D., Hinchliffe, S., Rahman, M. M., Bass, D., & Tyler, C. R. (2020). Evaluating antimicrobial resistance in the global shrimp industry. Reviews in aquaculture, 12(2), 966-986.
32. UDDIN, M. H., HASAN, M., AHASAN, M. M., HOSSAIN, M. A., & UDDIN, A. J. (2010). ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF TINOSPORA CORDIFOLIA. Int. J. Sustain. Agril. Tech, 6(12), 01-03.
33. Von Gadow, A., Joubert, E. and Hansmann, C. F. (1997). Comparison of the antioxidant activity of aspalathin with that of other plant phenols of rooibos tea (Aspalathus linearis), α-tocopherol, BHT, and BHA. Journal of agricultural and food chemistry, 45(3), 632-638.
34. Wagner, H. (1999). Immunomodulatory agents from plants. Springer Science & Business Media.
35. Yen, G. C. and Duh, P. D. (1994). Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. Journal of agricultural and food chemistry, 42(3), 629-632.
36. Yu, C., Tang, J., Liu, X., Ren, X., Zhen, M. and Wang, L. (2019). Green biosynthesis of silver nanoparticles using Eriobotrya japonica (Thunb.) leaf extract for reductive catalysis. Materials, 12(1), 189.
2. Babu, B., Sathiyaraj, G., Mandal, A., Kandan, S., Biju, N., Palanisamy, S., ... & Prabhu, N. M. (2021). Surveillance of disease incidence in shrimp farms located in the east coastal region of India and in vitro antibacterial efficacy of probiotics against Vibrio parahaemolyticus. Journal of invertebrate pathology, 179, 107536.
3. Benzie, I. F. and Strain, J. J. (1999). [2] Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. In Methods in enzymology (Vol. 299, pp. 15-27). Academic press.
4. Dar, W. (1999, March). Sustainable aquaculture development and the code of conduct for responsible fisheries. In The Ministerial Meeting on the Implementation of the Code of Conduct for Responsible Fisheries. Rome.
5. De La Pena, L. D., Lavilla-Pitogo, C. R. and Paner, M. G. (2001). Luminescent vibrios associated with mortality in pond-cultured shrimp Penaeus monodon in the Philippines: species composition. Fish Pathology, 36(3), 133-138.
6. Dhar, A. K., Piamsomboon, P., Caro, L. F. A., Kanrar, S., Adami Jr, R. and Juan, Y. S. (2019). First report of acute hepatopancreatic necrosis disease (AHPND) occurring in the USA. Diseases of aquatic organisms, 132(3), 241-247.
7. FAO, (2020). The State of World Fisheries and Aquaculture 2020. Sustainability in action, Rome. https://doi.org/10.4060/ca9229en (accessed on 22nd July 2020).
8. (FAO, (2016). The State of World Fisheries and Aquaculture 2016. Contributing to food security and nutrition for all. Rome. pp. 200.
9. Gulec, A. K., Altİnterİm, B. A. Ş. A. R. and Aksu, O. (2013). Determination of lethal concentration (LC50) values of Cinnamomum zeylanicum hydrosol on carp fish. Iranian Journal of Fisheries Sciences, 12(1), 34-44.
10. Holder, I. A. and Boyce, S. T. (1994). Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture. Burns, 20(5), 426-429.
11. Inglis, V. (2000). Antibacterial chemotherapy in aquaculture: review of practice, associated risks and need for action. In Use of Chemicals in Aquaculture in Asia: Proceedings of the Meeting on the Use of Chemicals in Aquaculture in Asia 20-22 May 1996, Tigbauan, Iloilo, Philippines (pp. 7-22). Aquaculture Department, Southeast Asian Fisheries Development Center.
12. Iswarya, A., Marudhupandi, T., Vaseeharan, B., Ibrahim, W. N. W., Leong, L. K., & Musa, N. (2022). Shrimp vibriosis. In Aquaculture pathophysiology (pp. 191-206). Academic Press.
13. Janarthanam, K., George, M. R., John, K. R. and Jeyaseelan, M. J. (2012). In vitro and in vivo biocontrol of Vibrio harveyi using indigenous bacterium, Bacillus spp.
14. Li, P., Kinch, L. N., Ray, A., Dalia, A. B., Cong, Q., Nunan, L. M. and Orth, K. (2017). Acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus strains maintain an antibacterial type VI secretion system with versatile effector repertoires. Applied and environmental microbiology, 83(13), e00737-17.
15. Marcocci, L., Maguire, J. J., Droylefaix, M. T., & Packer, L. (1994). The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochemical and biophysical research communications, 201(2), 748-755.
16. Modi, Bindu, Niranjan Koirala, Surya Prasad Aryal, Jiban Shrestha, Shishir Koirala, Jitendra Upadhyaya, Ram Chandra Basnyat, Mohamed A. Nassan, Mohammed Alqarni, and Gaber El-Saber Batiha. "Tinospora cordifolia (Willd.) Miers: phytochemical composition, cytotoxicity, proximate analysis and their biological activities." Cellular and Molecular Biology 67, no. 1 (2021): 50-57.
17. MPEDA, 2016. The Marine Product Development Authority (MPEDA) State-wise details of shrimp and scampi production. Accessed 13 February 2018. http://mpeda.gov.in.
18. Namrta Choudhary, N. C., Siddiqui, M. B., Shazia Azmat, S. A., & Sayyada Khatoon, S. K. (2013). Tinospora cordifolia: ethnobotany, phytopharmacology and phytochemistry aspects.
19. Pirtarighat, S., Ghannadnia, M. and Baghshahi, S. (2019). Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. Journal of Nanostructure in Chemistry, 9, 1-9.
20. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237..
21. Rhodes, G., Huys, G., Swings, J., Mcgann, P., Hiney, M., Smith, P., & Pickup, R. W. (2000). Distribution of oxytetracycline resistance plasmids between aeromonads in hospital and aquaculture environments: implication of Tn 1721 in dissemination of the tetracycline resistance determinant Tet A. Applied and environmental microbiology, 66(9), 3883-3890.
22. Roque, A., Turnbull, J. F., Escalante, G., Gomez-Gil, B. and Alday-Sanz, M. V. (1998). Development of a bath challenge for the marine shrimp Penaeus vannamei Boone, 1931. Aquaculture, 169(3-4), 283-290.
23. Saxena, C., & Rawat, G. (2019). Tinospora cordifolia (Giloy)-Therapeutic uses and importance: A review. Current Research in Pharmaceutical Sciences.
24. Sharma, P., Dwivedee, B. P., Bisht, D., Dash, A. K., & Kumar, D. (2019). The chemical constituents and diverse pharmacological importance of Tinospora cordifolia. Heliyon, 5(9).
25. Sinha, K., Mishra, N. P., Singh, J. and Khanuja, S. P. S. (2004). Tinospora cordifolia (Guduchi), a reservoir plant for therapeutic applications: A Review.
26. Song, J. Y., & Kim, B. S. (2009). Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess and biosystems engineering, 32, 79-84.
27. Sørum, H. (1999). Antibiotic resistance in Aquaculture. Acta Veterinaria Scandinavia, Supplementum, 92, 29–36.
28. Soto-Rodriguez, S. A., Gomez-Gil, B. and Lozano, R. (2010). ‘Bright-red’syndrome in Pacific white shrimp Litopenaeus vannamei is caused by Vibrio harveyi. Diseases of aquatic organisms, 92(1), 11-19.
29. Tacon, A. G. (2020). Trends in global aquaculture and aquafeed production: 2000–2017. Reviews in Fisheries Science & Aquaculture, 28(1), 43-56.
30. Teng, T., Liang, L., Chen, K., Xi, B., Xie, J. and Xu, P. (2017). Isolation, identification and phenotypic and molecular characterization of pathogenic Vibrio vulnificus isolated from Litopenaeus vannamei. PLoS One, 12(10), e0186135.
31. Thornber, K., Verner‐Jeffreys, D., Hinchliffe, S., Rahman, M. M., Bass, D., & Tyler, C. R. (2020). Evaluating antimicrobial resistance in the global shrimp industry. Reviews in aquaculture, 12(2), 966-986.
32. UDDIN, M. H., HASAN, M., AHASAN, M. M., HOSSAIN, M. A., & UDDIN, A. J. (2010). ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF TINOSPORA CORDIFOLIA. Int. J. Sustain. Agril. Tech, 6(12), 01-03.
33. Von Gadow, A., Joubert, E. and Hansmann, C. F. (1997). Comparison of the antioxidant activity of aspalathin with that of other plant phenols of rooibos tea (Aspalathus linearis), α-tocopherol, BHT, and BHA. Journal of agricultural and food chemistry, 45(3), 632-638.
34. Wagner, H. (1999). Immunomodulatory agents from plants. Springer Science & Business Media.
35. Yen, G. C. and Duh, P. D. (1994). Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. Journal of agricultural and food chemistry, 42(3), 629-632.
36. Yu, C., Tang, J., Liu, X., Ren, X., Zhen, M. and Wang, L. (2019). Green biosynthesis of silver nanoparticles using Eriobotrya japonica (Thunb.) leaf extract for reductive catalysis. Materials, 12(1), 189.
Published
2024-07-30
How to Cite
Ankesh Yadav, Ragunathan M.G, & Jayanthi.J. (2024). In Vivo, In Vitro Rt-Pcr Based Gene Expression Studies On Biological Activities Of Biosynthesized Silver Nanoparticles From Tinospora Cordifolia (Thunb.) Plant Extract Against Vibriosis Infection In The Shrimp Litopenaeus vannamei (Boone, 1931). Revista Electronica De Veterinaria, 25(1), 659-675. Retrieved from https://veterinaria.org/index.php/REDVET/article/view/632
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