In vitro studies of Withania somnifera plant extract with selected Antibiotics
Keywords:
Withania somnifera Extract, HPTLC, Antibiotics, Antimicrobial Activity
Abstract
Antibiotics play a major role in clinical medicine in eradicating pathogens. The arrival of genuine strains of bacteria has seriously limited our talent to treat bacteria and selected antibiotics are dreadfully desirable. In this study, the plant extracts were prepared by using diff. solvents (Acetone/Ethanol/Methanol). Antimicrobial evaluation of plant extracts, some particular Antibiotics like Amoxicillin, Ceftazidime, Ciprofloxacin, Amphotericin-B and Fluconazole and combinations has been done by Agar well Diffusion method. The results of this study displayed that ethanol and methanol extract show higher activity against nominated pathogenic species. HPTLC has been also achieved to check if there any new compound formed or not by amalgamation of extract and antibiotics. The findings of this study suggest that coalescing plant extract with antibiotics may be effective in battling newly developed drug-resistant microbes.
References
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23. Burra, D. D., Mühlenbock, P., & Andreasson, E. (2015). Salicylic and jasmonic acid pathways are necessary for defence against D ickeya solani as revealed by a novel method for blackleg disease screening of in vitro grown potato. Plant Biology, 17(5), 1030-1038.
24. Khan, M. A., Zhao, Y., & Korban, S. S. (2012). Molecular mechanisms of pathogenesis and resistance to the bacterial pathogen Erwinia amylovora, causal agent of fire blight disease in Rosaceae. Plant Molecular Biology Reporter, 30, 247-260.
2. Kulkarni, S. K., & Dhir, A. (2008). Withania somnifera: an Indian ginseng. Progress in neuro-psychopharmacology and biological psychiatry, 32(5), 1093-1105.
3. Archana, R., & Namasivayam, A. (1998). Antistressor effect of Withania somnifera. Journal of ethnopharmacology, 64(1), 91-93.
4. Umadevi, M., Rajeswari, R., Rahale, C. S., Selvavenkadesh, S., Pushpa, R., Kumar, K. S., & Bhowmik, D. (2012). Traditional and medicinal uses of Withania somnifera. The pharma innovation, 1(9, Part A), 102.
5. Dar, N. J., Hamid, A., & Ahmad, M. (2015). Pharmacologic overview of Withania somnifera, the Indian Ginseng. Cellular and molecular life sciences, 72, 4445-4460.
6. Bhattacharya, S. K., Satyan, K. S., & Ghosal, S. (1997). Antioxidant activity of glycowithanolides from Withania somnifera. Indian journal of experimental biology, 35(3), 236-239.
7. Bhattacharya, S. K., Goel, R. K., Kaur, R., & Ghosal, S. (1987). Anti‐stress activity of sitoindosides VII and VIII, new acylsterylglucosides from Withania somnifera. Phytotherapy research, 1(1), 32-37.
8. Dhanani, T., Shah, S., Gajbhiye, N. A., & Kumar, S. (2017). Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera. Arabian journal of chemistry, 10, S1193-S1199.
9. Rai, M., Jogee, P. S., Agarkar, G., & Santos, C. A. D. (2016). Anticancer activities of Withania somnifera: Current research, formulations, and future perspectives. Pharmaceutical biology, 54(2), 189-197.
10. Sharifi-Rad, J., Quispe, C., Ayatollahi, S. A., Kobarfard, F., Staniak, M., Stępień, A., ... & Cho, W. C. (2021). Chemical Composition, Biological Activity, and Health‐Promoting Effects of Withania somnifera for Pharma‐Food Industry Applications. Journal of Food quality, 2021(1), 8985179.
11. Tewari, D., Chander, V., Dhyani, A., Sahu, S., Gupta, P., Patni, P., ... & Bishayee, A. (2022). Withania somnifera (L.) Dunal: Phytochemistry, structure-activity relationship, and anticancer potential. Phytomedicine, 98, 153949.
12. Munir, N., Mahmood, Z., Shahid, M., Afzal, M. N., Jahangir, M., Ali Shah, S. M., ... & Yousaf, F. (2022). Withania somnifera chemical constituents’ in vitro antioxidant potential and their response on spermatozoa parameters. Dose-Response, 20(1), 15593258221074936.
13. Kumar, V., Dey, A., Hadimani, M. B., Marcovic, T., & Emerald, M. (2015). Chemistry and pharmacology of Withania somnifera: An update. CellMed, 5(1), 1-1.
14. Singh, G., & Kumar, P. (2014). Antibacterial activity of flavonoids of Withania somnifera L. International Journal of Green Pharmacy (IJGP), 8(2).
15. Khanna, P. K., Kumar, A., Ahuja, A., & Kaul, M. K. (2007). Fatty Acid Composition of Seed Oil of Withania somnifera. Physiol. Mol. Biol. Plants, 13(1), 73-76.
16. Kumar, B., Bajpai, V., Gond, V., Tiwari, S., & Madhusudanan, K. P. (2021). Phytochemistry of Withania somnifera. CRC Press.
17. Owais, M., Sharad, K. S., Shehbaz, A., & Saleemuddin, M. (2005). Antibacterial efficacy of Withania somnifera (ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis. Phytomedicine, 12(3), 229-235.
18. A Dar, P., R Singh, L., A Kamal, M., & A Dar, T. (2016). Unique medicinal properties of Withania somnifera: Phytochemical constituents and protein component. Current pharmaceutical design, 22(5), 535-540.
19. Golanowska, M., Kielar, J., & Lojkowska, E. (2017). The effect of temperature on the phenotypic features and the maceration ability of Dickeya solani strains isolated in Finland, Israel and Poland. European Journal of Plant Pathology, 147, 803-817.
20. Golanowska, M., & Lojkowska, E. (2016). A review on Dickeya solani, a new pathogenic bacterium causing loss in potato yield in Europe. BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology, 97(2).
21. van der Wolf, J. M., Cahill, G., Van Gijsegem, F., Helias, V., Humphris, S., Li, X., ... & Pritchard, L. (2021). Isolation, detection and characterization of Pectobacterium and Dickeya Species. Plant Diseases Caused by Dickeya and Pectobacterium Species, 149-173.
22. Golanowska, M., & Lojkowska, E. (2016). A review on Dickeya solani, a new pathogenic bacterium causing loss in potato yield in Europe. BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology, 97(2).
23. Burra, D. D., Mühlenbock, P., & Andreasson, E. (2015). Salicylic and jasmonic acid pathways are necessary for defence against D ickeya solani as revealed by a novel method for blackleg disease screening of in vitro grown potato. Plant Biology, 17(5), 1030-1038.
24. Khan, M. A., Zhao, Y., & Korban, S. S. (2012). Molecular mechanisms of pathogenesis and resistance to the bacterial pathogen Erwinia amylovora, causal agent of fire blight disease in Rosaceae. Plant Molecular Biology Reporter, 30, 247-260.
Published
2023-02-11
How to Cite
Niharika Jegoda, Vikram Panchal, Namrata Patel, Kinjal Patel, & Piyush J. Vyas. (2023). In vitro studies of Withania somnifera plant extract with selected Antibiotics. Revista Electronica De Veterinaria, 24(2), 535-540. https://doi.org/10.69980/redvet.v24i2.652
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