In Vitro Evaluation of the Phytochemical Components of New Multi-Herbal Medicine LIV01-SSA-23
Abstract
The active ingredients of plants ware identified using a suitable solvent, the solutions were evaporated, and the residues were then adjusted to a set as standard to create polyherbal formulations, which were concentrated herbal compounds. The main components of LIV01-SSA-23 were the Indian herbs Andrographis paniculata, Moringa oleifera, Ocimum Sanctum, Withania somnifera, Tinospora cordifolia, Picrorrhiza kurroa, and Terminalia chebula, which was excellent for treating a variety of liver problems and preventing oxidative cellular damage. Phytochemicals that have the potential to treat diseases and were used in natural therapeutics include flavonoids, glycosylates, saponins, amino acids, monoterpenes, and others. The goal of this inquiry was to identify the many phytochemicals that were present in the extracts included in LIV01-SSA-23. Several phytochemical components were examined using previously standardized procedures. Saponins, flavonoids, steroids, terpenoids, triterpenoids, alkaloids, sugars, anthraquinone, phenolic compounds, and glycosides were all present in the phytochemical sample. Using ethanol, methanol, and aqueous solutions, the substances ware extracted. Saponins, flavonoids, steroids, terpenoids, triterpenoids, alkaloids, carbohydrates, anthraquinone, phenolic compounds, and glycosides were found to be the phytochemical constituents of the formulation. For analytical study, criteria such as loss on drying, total ash, acid-soluble ash, and pH, the pharmacopeial constraints were satisfied. According to preliminary phytochemical screening of LIV01-SSA-23, the presence of active components was found. These active group of compounds which were found responsible for the therapeutic properties important for pharmacological action of the formulation.
References
2. Farnsworth NR, Akerele O, Bingle AS, SojartoDD,Guo Z. Medicinal plant in therapy. Bulletin of the world health organization. 1985; 63:965-981.
3. Abraham R, Paridhavi M. A review of comprehensive study on medicinal plants of polyherbal formulation-Churna. Asian J Pharm Clin Res 2013; 6(4):11-18.
4. Kunwar RM, Shrestha KP, Bussmann RW. Traditional herbal medicine in far-west Nepal: a pharmacological appraisal. J EthnobiolEthnomed 2010; 6:35.
5. Ngo LT, Okogun JI, Folk WR. 21st century natural product research and drug development and traditional medicines. Nat Prod Rep 2014; 30(4):584-92.
6. Bhope SG, Nagore DH, Kuber VV, Gupta PK, Patil MJ. Design and development of a stable polyherbal formulation based on the results of compatibility studies, Pharmacognosy Res. 2011;3(2): 122-129.
7. Bhattacharya SK, Kumar A. Effect of Trasina, an ayurvedic herbal formulation, on experimental models of Alzheimer's disease and central cholinergic markers in rats. J Altern Complement Med. 1997; 3(4):327-336.
8. Bhattacharya SK, Ghosal S. Effect of Shilajit on rat brain monoamines. Phytotherapy Research. 1992; 6(3): 163 – 164.
9. Darbar S,Chattopadhyay SP. Acute oral toxicity study of Trasina®, an Ayurvedic herbal formulation on experimental models. J. Pharm. Med. Res. 2019; 4(1): 84-86
10. Darbar S, Saha S, Chattopadhyay S, Chattapadhyay A, Anti-Stress Activity (in-vivo) of Multi Herbal Capsule-Trasina® in Experimental Murine Model, Asian Journal of Pharmaceutical Research and Development. 2020; 8(5):52-58
11. Darbar Soumendra and Roy Biswanath. Evaluation of accelerated stability study of ayurvedic formulation – Trasina®. International Journal of Research in Pharmacy and Pharmaceutical Sciences. 2023: 8(2); 38-41.
12. Rohini MV, Padmini E. Preliminary phytochemical screening of selected medicinal plants of polyherbal formulation. Journal of Pharmacognosy and Phytochemistry. 2016;5(5):277-82.
13. Mekala P, Murthy TG. Phytochemical screening and pharmacological update on KabasuraKudineerchoornam and NilavembuKudineerChoornam. Journal of Pharmacognosy and Phytochemistry. 2020;9(3):1031-6.
14. Yadav M, Chatterji S, Gupta SK, Watal G. Preliminary phytochemical screening of six medicinal plants used in traditional medicine. Int J Pharm Pharm Sci. 2014;6(5):539-42.
15. Agarwal S, Darbar S, Saha S, Deb T. Sanative effect of a low-cost Novel Green Formulation –IM-SSS20 to minimize the inflammatory and cytokine storm against Respiratory Diseases. Innoriginal: International Journal of Sciences. 2020 Nov 22:1-1.
16. Darbar S, Saha S, Pramanik K, Chattopadhyay A. Antioxidant and immunomodulatory effect of AKSS16-LIV01–a multi herbal formulation against ethanol induced liver dysfunction in mice. Clinical Phytoscience. 2021 Dec;7(1):1-20.
17. Panduranga Murthy G, Harsha Ramakrishna, Sushma S. Murthy, Divya R, MamathaRani
D.R. Hydroxy radical and DPPH scavenging activity of crude protein extract of Leucas
linifolia: A folk medicinal plant. Asian Journal of Plant Science and Research, 2012;
2(1): 30-35.
18. M. R. Sahaa, S. M. R. Hasana, R. Aktera, M. M. Hossaina, M. S. Alamb, M. A., and M.
E. H. Mazumderc. In Vitro free Radical Scavenging Activity Of Methanol Extract Of
The Leaves Of MimusopsElengi Linn. Bangl. J. Vet. Med, 2008; 6(2): 197–202.
19. Mandal S, Patra A, Samanta A, Roy S, Mandal A, Mahapatra TD, Pradhan S, Das K, Nandi DK. Analysis of phytochemical profile of Terminalia arjuna bark extract with antioxidative and antimicrobial properties. Asian Pacific journal of tropical biomedicine. 2013 Dec 1;3(12):960-6.
20. Singh R, Singh SK, Arora S. Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn. Fod Chem. Toxicol. 2007; 45:1216-1223.
21. Han X, Shen T, Lou H. Dietery polyphenols and their biological significance. Int. J. Mol. Sci. 2007, 950-988.
22. Brown JE, Rice-Evans CA. Luteolin rich artichoke extract protects low density lipoprotein from oxidation in vitro. Free Radical Res. 1998; 29:247-255.
23. Ali SS, Kasoju N, Luthra A, Singh A, Sharanabasava H, Sahuand A et al. Indian medicinal herbs as source of antioxidants. Food Res. Int., 2008; 41:1-15.
24. Marjorie C. Plant products as antimicrobial agents. ClincalMicrobiol. Rev. 1996; 12:564-582.
25. Salah N, Miller NJ, Pagange G, Tijburg L, Bolwell GP, Rice E et al. Polyphenolic flavonoids as scavenger of aqueous phase radicals as chai breaking antioxidant. Arc. Biochem. Broph. 1995; 2:339-346.
26. Del-Rio A, Obdululio BG, Casfillo J, Main FG, Ortuno A. Uses and properties of citrus flavonoids. J. Agric. Food Chem. 1997; 45:4505-4515.
27. Okwu DE. Phytochemicals and vitamin content of indigenous species of southeastern Nigeria. J. Sustain. Agric. Environ., 2004; 6(1):30-37.
