Antimicrobial Resistance Patterns in Companion Animals: A Multicentre Study
Abstract
The objective of this multicentre study was to evaluate the prevalence of AMR in companion animals in different geographical locations. Carried out in conjunction with the veterinary practices and diagnostic laboratories, the survey involved 1,200 animals: 800 dogs and 400 cats with bacterial infections. Specimens such as skin swabs, urine, blood and ear swabs were taken and cultured and identified and their susceptibility to antimicrobials was determined. The average resistance rate was 48% and it was also observed that there are disparities in the different regions. Resistance was higher in the urban regions at 52% as compared to the rural regions at 43%. Resistance patterns were different with the species and higher resistance was observed in dogs (50%) as compared to cats (45%). The following specific pathogens showed significant resistance: Staphylococcus pseudintermedius and Escherichia coli. For example, S. pseudintermedius was 60% resistant to methicillin; E. coli was 50% resistant to ampicillin. The study further showed that AMR rates differ significantly between centres (p < 0. 05) and there are correlations between AMR and animals’ attributes. These results highlight the necessity of developing the regional surveillance of AMR and the targeted interventions in companion animals.
References
2. European Food Safety Authority. (2015). The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals, and food in 2013. EFSA Journal, 13(2), 4036.
3. Ewers, C., Grobbel, M., Bethe, A., Wieler, L. H., Guenther, S., & Schwarz, S. (2010). Extended-spectrum β-lactamases-producing gram-negative bacteria in companion animals: action is clearly warranted!. Berliner und Munchener tierarztliche Wochenschrift, 123(3-4), 111-112.
4. Guardabassi, L., Schwarz, S., & Lloyd, D. H. (2004). Pet animals as reservoirs of antimicrobial-resistant bacteria. Journal of Antimicrobial Chemotherapy, 54(2), 321-332.
5. Laxminarayan, R., Duse, A., Wattal, C., Zaidi, A. K., Wertheim, H. F., Sumpradit, N., ... & Cars, O. (2013). Antibiotic resistance—the need for global solutions. The Lancet infectious diseases, 13(12), 1057-1098.
6. Lloyd, D. H. (2007). Reservoirs of antimicrobial resistance in pet animals. Clinical Infectious Diseases, 45(Supplement_2), S148-S152.
7. American Veterinary Medical Association (AVMA). (2019). AVMA Guidelines for the Depopulation of Animals: 2019 Edition. Retrieved from https://www.avma.org/sites/default/files/resources/AVMA-Guidelines-for-the-Depopulation-of-Animals.pdf
8. Clinical and Laboratory Standards Institute (CLSI). (2020). Performance Standards for Antimicrobial Susceptibility Testing, 30th Edition. Retrieved from https://clsi.org/standards/products/microbiology/documents/m100/
9. Guardabassi, L., Schwarz, S., & Lloyd, D. H. (2004). Pet animals as reservoirs of antimicrobial-resistant bacteria. Journal of Antimicrobial Chemotherapy, 54(2), 321-332. https://doi.org/10.1093/jac/dkh332
10. International Organization for Standardization (ISO). (2019). ISO 15189:2012 Medical laboratories — Requirements for quality and competence. Retrieved from https://www.iso.org/standard/56115.html
11. Laxminarayan, R., Duse, A., Wattal, C., Zaidi, A. K., Wertheim, H. F., Sumpradit, N., ... & Cars, O. (2013). Antibiotic resistance—the need for global solutions. The Lancet infectious diseases, 13(12), 1057-1098. https://doi.org/10.1016/S1473-3099(13)70318-9
12. O'Neill, J. (2014). Antimicrobial resistance: tackling a crisis for the health and wealth of nations. Review on Antimicrobial Resistance.
13. One Health Commission. (2020). What is One Health? Retrieved from https://www.onehealthcommission.org/en/why_one_health/what_is_one_health/
14. Prescott, J. F., & Boerlin, P. (2016). Antimicrobial use in companion animals and the emergence of resistance. The Canadian Veterinary Journal, 57(2), 135-144.
15. Weese, J. S., & van Duijkeren, E. (2010). Methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in veterinary medicine. Veterinary microbiology, 140(3-4), 418-429.
16. World Health Organization. (2020). Antimicrobial resistance. Retrieved from https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance
17. American Veterinary Medical Association (AVMA). (2019). AVMA Guidelines for the Depopulation of Animals: 2019 Edition. Retrieved from https://www.avma.org/sites/default/files/resources/AVMA-Guidelines-for-the-Depopulation-of-Animals.pdf
18. Clinical and Laboratory Standards Institute (CLSI). (2020). Performance Standards for Antimicrobial Susceptibility Testing, 30th Edition. Retrieved from https://clsi.org/standards/products/microbiology/documents/m100/
19. Guardabassi, L., Schwarz, S., & Lloyd, D. H. (2004). Pet animals as reservoirs of antimicrobial-resistant bacteria. Journal of Antimicrobial Chemotherapy, 54(2), 321-332. https://doi.org/10.1093/jac/dkh332
20. International Organization for Standardization (ISO). (2019). ISO 15189:2012 Medical laboratories — Requirements for quality and competence. Retrieved from https://www.iso.org/standard/56115.html
21. Laxminarayan, R., Duse, A., Wattal, C., Zaidi, A. K., Wertheim, H. F., Sumpradit, N., ... & Cars, O. (2013). Antibiotic resistance—the need for global solutions. The Lancet infectious diseases, 13(12), 1057-1098. https://doi.org/10.1016/S1473-3099(13)70318-9.
