In Vitro Propagation of Eryngium Foetidum L. Via Callus Induction And Multiple Shoot Formation
Keywords:
in vitro propagation, callus induction, nodal segments, E. foetidum, multiple shoots, Growth regulators
Abstract
A reliable and straightforward procedure for in vitro propagation of Eryngium foetidum was established using nodal and leaf explants. This study was conducted to evaluate the consequences of various growth regulators on callus induction and multiple shoot development. Callus induction was achieved at a rate of 100% when the medium was supplemented with IAA (5.7 µM). Meanwhile, multiple shoots were produced at an average of 6 ± 0.31 per plantlet when the medium was supplemented with a combination of BA (1.11µM) and Kinetin (1.16 µM). Following this, the plantlets were successfully hardened and acclimatized in the field with 70% survival rate.
References
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2. Arockiasamy S, Prakash S and Ignacimuthu S (2002) Direct organogenesis from mature leaves and petioles of Eryngium foetidum L. Biol. Plant., 45:129–132.
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5. David Raja H and Arockiasamy DI (2008) In vitro Propagation of Mentha viridis L. from Nodal and Shoot tip Explants. Plant.Tissue.Cult.Biotech., 18:1-6.
6. Dode LB, Bobrowski VL, Braga EJB, Seixas FK and Schuch MW (2003) Invitro propagation of Ocimum basilicum L. (Lamiaceae).ActaScientiarum Biol. Sci., 25(2): 435-437.
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8. Ignacimuthu S, Arockiasamy S, Antonysamy M and Ravichandran P (1999) Plant regeneration through somatic embryogenesis from mature leaf explants of Eryngium foetidum, a condiment. Plant Cell Tiss.Organ Cult. 56:131–137.
9. KaushalSood and Yadav RNS (2014) Effect of solvent on the extraction of Antioxidant and Antimicrobial components from Eryngium foetidum L. International Journal of Medicine and Pharmaceutical Sciences (IJMPS), 4 (5): 31-40.
10. Kiran GCP, Kaviraj RB, Venugopal FTZ and Rao S (2004) Rapid regeneration of Mentha piperita L. from shoots tip and nodal explants. Indian J. Biotech. 3:594-598.
11. Lingaraju, Sudarshana MS, Mahendra C and PoornachandraRao K (2016) Phytochemical screening and Antimicrobial activity of leaf extracts of Eryngium foetidum L. (Apiaceae).Indo American Journal of Pharmaceutical Research,6 (2): 4339-4344
12. Martin K P (2002) Rapid propagation of HolostemmaadaKodienSchul., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis. Plant Cell Reports, 21: 112-117.
13. Martin KP (2003) In vitro propagation of the herbal spice Eryngium foetidum L. on sucrose added and sucrose-free medium without growth regulators and CO2 enrichment. ScientiaHorticulturae, 102: 277–282.
14. Nirmal KS and Sehgal CB (1999) Micropropagation of ‘Holy Basil’ (Ocimum sanctum Linn) from young inflorescences of mature plants. Plant Growth Regulation, 29(3): 161-166.
15. Paula JHA, Seafortha CE and Tikasinghb T (2011) Eryngium foetidum L A review. Fitoterapia; 82 (3): 302-308.
16. Rani G, Virk GS and Nagpal A (2003) Callus induction and plantlet regeneration in Withania somnifera (L) Dunal. In vitro Cellular & Developmental Biology Plant., 39(5):468-474.
17. Saenz MT, Fernandez MA and Garcia MD (1997) Antiinflammatory and analgesic properties from leaves of EryngiumfoetidumL. (Apiaceae). Phytother. Res. 11:380–383.
18. SanghamitraSamantaray, Ashok Kumar Bishoyi and SatyabrataMaiti (2013) Plant regeneration from callus cultures of Vitextrifolia (Lamiales:Lamiaceae): a potential medicinal plant.Revista de biologia tropical, 61(3):1083-94
19. Sankat CK and Maharaj V (1991) Refrigerated storage of shadobenni (Eryngium foetidum L) In: Proceedings of Agriculture Institute of Canada annual conference, Fredericton, New Brunswick, Canada.
20. Sreekumar S, Seeni S and Pushpangadan P(2000) Micropropagation of Hemidesmus indicus for cultivation and production of 2- hydroxyl 4-methoxy benzaldehyde. Plant Cell, Tissue and Organ Culture, 62: 211- 218.
21. Thomas TD and Maseena EA (2006) Callus induction and plant regeneration cardiospermum halicacabum Linn an important medicinal plant. Scientia Horticulturae, 108(3):332-336.
22. Yasseen (2002) In vitro regeneration, flower and plant formation from petiolar and nodal explants of culantro (Eryngium foetidum L) In Vitro Cell. Dev. Biol.—Plant 38:423–426.
2. Arockiasamy S, Prakash S and Ignacimuthu S (2002) Direct organogenesis from mature leaves and petioles of Eryngium foetidum L. Biol. Plant., 45:129–132.
3. Burkill IH (1966) A dictionary of the economic products of the Malay peninsula, 2nd edition, Government of Malaysia and Singapore, Kuala Lumpur, Pages 110.
4. Chandrika R, Shivakameshwari MN and TharaSaraswathi KJ (2015) Reduction of vitrification in In vitro shoot cultures of Eryngium foetidum L. – A potential Aromatic and Medicinal herb. Indian Journal of Plant Sciences, 4 (2): 2-58.
5. David Raja H and Arockiasamy DI (2008) In vitro Propagation of Mentha viridis L. from Nodal and Shoot tip Explants. Plant.Tissue.Cult.Biotech., 18:1-6.
6. Dode LB, Bobrowski VL, Braga EJB, Seixas FK and Schuch MW (2003) Invitro propagation of Ocimum basilicum L. (Lamiaceae).ActaScientiarum Biol. Sci., 25(2): 435-437.
7. Gupta SK, Khaneja SPS and Kumar S (2007) In vitro propagation of Lippia alba. Current science, 81: 206-210.
8. Ignacimuthu S, Arockiasamy S, Antonysamy M and Ravichandran P (1999) Plant regeneration through somatic embryogenesis from mature leaf explants of Eryngium foetidum, a condiment. Plant Cell Tiss.Organ Cult. 56:131–137.
9. KaushalSood and Yadav RNS (2014) Effect of solvent on the extraction of Antioxidant and Antimicrobial components from Eryngium foetidum L. International Journal of Medicine and Pharmaceutical Sciences (IJMPS), 4 (5): 31-40.
10. Kiran GCP, Kaviraj RB, Venugopal FTZ and Rao S (2004) Rapid regeneration of Mentha piperita L. from shoots tip and nodal explants. Indian J. Biotech. 3:594-598.
11. Lingaraju, Sudarshana MS, Mahendra C and PoornachandraRao K (2016) Phytochemical screening and Antimicrobial activity of leaf extracts of Eryngium foetidum L. (Apiaceae).Indo American Journal of Pharmaceutical Research,6 (2): 4339-4344
12. Martin K P (2002) Rapid propagation of HolostemmaadaKodienSchul., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis. Plant Cell Reports, 21: 112-117.
13. Martin KP (2003) In vitro propagation of the herbal spice Eryngium foetidum L. on sucrose added and sucrose-free medium without growth regulators and CO2 enrichment. ScientiaHorticulturae, 102: 277–282.
14. Nirmal KS and Sehgal CB (1999) Micropropagation of ‘Holy Basil’ (Ocimum sanctum Linn) from young inflorescences of mature plants. Plant Growth Regulation, 29(3): 161-166.
15. Paula JHA, Seafortha CE and Tikasinghb T (2011) Eryngium foetidum L A review. Fitoterapia; 82 (3): 302-308.
16. Rani G, Virk GS and Nagpal A (2003) Callus induction and plantlet regeneration in Withania somnifera (L) Dunal. In vitro Cellular & Developmental Biology Plant., 39(5):468-474.
17. Saenz MT, Fernandez MA and Garcia MD (1997) Antiinflammatory and analgesic properties from leaves of EryngiumfoetidumL. (Apiaceae). Phytother. Res. 11:380–383.
18. SanghamitraSamantaray, Ashok Kumar Bishoyi and SatyabrataMaiti (2013) Plant regeneration from callus cultures of Vitextrifolia (Lamiales:Lamiaceae): a potential medicinal plant.Revista de biologia tropical, 61(3):1083-94
19. Sankat CK and Maharaj V (1991) Refrigerated storage of shadobenni (Eryngium foetidum L) In: Proceedings of Agriculture Institute of Canada annual conference, Fredericton, New Brunswick, Canada.
20. Sreekumar S, Seeni S and Pushpangadan P(2000) Micropropagation of Hemidesmus indicus for cultivation and production of 2- hydroxyl 4-methoxy benzaldehyde. Plant Cell, Tissue and Organ Culture, 62: 211- 218.
21. Thomas TD and Maseena EA (2006) Callus induction and plant regeneration cardiospermum halicacabum Linn an important medicinal plant. Scientia Horticulturae, 108(3):332-336.
22. Yasseen (2002) In vitro regeneration, flower and plant formation from petiolar and nodal explants of culantro (Eryngium foetidum L) In Vitro Cell. Dev. Biol.—Plant 38:423–426.
Published
2024-09-24
How to Cite
Sahaya Shibu, Agila Gopinath, Sarmad Moin, & Servin Wesley. (2024). In Vitro Propagation of Eryngium Foetidum L. Via Callus Induction And Multiple Shoot Formation. Revista Electronica De Veterinaria, 25(1S), 1067 -1071. https://doi.org/10.69980/redvet.v25i1S.994
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Articles
