Green synthesis, Characterization and Anticancer activityof Clerodendrum infortunatum mediated ZrO2 and Ag doped ZrO2 nanoparticles
Keywords:
Green synthesis; Nanoparticles; Clerodendrum infortunatum leaves; Anticancer activity; Cell viability
Abstract
Clerodendrum infortunatum extract was employed for the green synthesis of pure ZrO2 and Ag‑doped ZrO2 nanoparticles. The study aimed to evaluate their anticancer efficacy against AGS (human gastric adenocarcinoma) cells. The nanoparticles were characterized by XRD, EDS, TEM, XPS, UV‑visible spectroscopy, and photoluminescence. Cytotoxicity assays (MTT) on AGS cells showed that both pure ZrO2 and Ag‑doped ZrO2, as well as the plant extract alone, induced cell death, with the doped sample exhibiting significantly stronger activity. Consequently, the biogenic nanoparticles qualify as promising anticancer agents for biomedical applications.
References
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28. S. Iqbal, M. Javed, A. Bahadur, M.A. Qamar, M. Ahmad, M. Shoaib, M. Raheel, N. Ahmad, M.B. Akbar and H. Li, Controlled synthesis of Ag doped CuO nanoparticles as a core with poly(acrylic acid) microgel shell for efcient removal of methylene blue under visible light, Journal of Materials Science: Materials in Electronics, 31 (2020) 8423-8432.
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31. M. Mohammadikish and A.A. Akradi, Synthesis and optical band gap determination of CuO nanoparticles from salen-based infinite coordination polymer nanospheres, Material Research Express, 6 (2019) 045013.
32. J. Samuel, J. E. shaji, S. S. J. Dhas, S. Suresh, V. S. Vinita and C.S.Biju, UV-blocking performance and antibacterial activity of Cd, Ba co-doped ZnO nanomaterials prepared by a facile wet chemical method, Surface and Interface Analysis, 1 (2023) 1-5.
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40. M. Ahamed, M. J. Akhtar, M. A. M. Khan and H. A. Alhadlaq, Enhanced Anticancer Performance of Eco-Friendly-Prepared MoZnO/RGO Nanocomposites: Role of Oxidative Stress and Apoptosis, ACS Omega, 7 (2022) 7103.
41. R. Rajendran and A. Mani, Photocatalytic, antibacterial and anticancer activity of silver-doped zinc oxide nanoparticles, Journal of Saudi Chemical Society, 24 (2020) 1010–1024.
42. N. Lewinski, V. Colvin and R. Drezek, Cytotoxicity of Nanoparticles, small, 4 (2008) 26–49.
2. P.Jakinala, H.N. Lavudi, N. Angali, S. Ganderla, K.K. Inampudi, S.B. Andugulapati,M. Srinivas and M.R. Katika, Green synthesis of ZnO-Ag nanocomposite using Stenotaphrumsecundatum grass extract: Antibacterial activity and anticancer effect in oral squamous cell carcinoma CAL 27 cells, Inorganic Chemistry Communications, 152 (2023) 110735-110741.
3. D.R.A. Preethi, S. Prabhu, V. Ravikumar and A. Philominal, Anticancer activity of pure and silver doped copper oxide nanoparticles against A549 Cell line, Materials Today Communication, 33 (2022) 104462-104467.
4. R. G. Chaudhary, A. K. Potbhare, P. B. Chouke, and A. R. Rai, Graphene-based materials and their nanocomposites with metal oxides: biosynthesis, electrochemical, photocatalytic and antimicrobial applications, Materials Research Foundations, 83 (2020) 79–116.
5. X. Qu, D. Zhou, J. Lu, D. Qin, J. Zhou, and H.-J. Liu, Cancer nanomedicine in preoperative therapeutics: nanotechnologyenabled neoadjuvant chemotherapy, radiotherapy, immunotherapy, and phototherapy, Bioactive Materials, 24 (2023) 136–152.
6. S. Dubey, T. Virmani, S. K. Yadav, A. Sharma, G. Kumar and A. Alhalmi, Breaking Barriers in Eco-Friendly Synthesis of Plant-Mediated Metal/Metal Oxide/Bimetallic Nanoparticles: Antibacterial, Anticancer, Mechanism Elucidation, and Versatile Utilizations, Journal of Nanomaterials, 2024 (2024).
7. S. Balaji, B.K. Mandal, S. Ranjan, N. Dasgupta and R. Chidambaram, Nano-zirconia – Evaluation of its antioxidant and anticancer activity, Journal of Photochemistry and Photobiology B: Biology, 170 (2017) 125-131.
8. S. Alanazi, Z. M. Alaizeri, R. Lateef, N. Madkhali, A. Alharbi and M. Ahamed, Zn Doping Improves the Anticancer Efficacy of SnO2 Nanoparticles, Applied Sciencese, 13 (2023) 12456-12462.
9. Y. Mbenga, J.O. Adeyemi, D.M.N. Mthiyane, M. Singh and D.C. Onwudiwe, Green synthesis, antioxidant and anticancer activities of TiO2 nanoparticles using aqueous extract of Tulbhagia violacea, Results in Chemistry, 6 (2023) 101007-101012.
10. A. Fakhri, S. Behrouz, I. Tyagi, S. Agarwal and V. K. Gupta, Synthesis and characterization of ZrO2 and carbon-doped ZrO2 nanoparticles for photocatalytic application, Journal of Molecular Liquids, 216 (2016) 342-346.
11. A. Annu, C. Sivasankari and U. Krupasankar, Synthesis and characerization of Zro2 nanoparticle by leaf extract bioreduction process for its biological studies. Mater Today Procceding, 33 (2020) 5317–5323.
12. C. Dhandapani, R. Narayanasamy and S. N. Karthick, Drastic photocatalytic degradation of methylene blue dye by neodymium doped zirconium oxide as photocatalyst under visible light irradiation, Optik, 127 (2016) 10288–10296.
13. T. V. Tran, D. T. C. Nguyen, P. S. Kumar, A. T. M. Din, A. A. Jalil and D. V. N. Vo, Green synthesis of ZrO2 nanoparticles and nanocomposites for biomedical and environmental applications: a review, Environmental Chemistry Letters, 20 (2022) 1309–1331.
14. S. Raj, M. Hattori and M. Ozawa, Ag-doped ZrO2 nanoparticles prepared by hydrothermal method for efficient diesel soot oxidation, Materials Letters, 234 (2019) 205–207.
15. K. Parveen, V. Banse and L. Ledwani, Green synthesis of nanoparticles: Their advantages and disadvantages, American institute of Physics, 1724 (2016) 020048-20053.
16. F. Ameen, Optimization of the synthesis of fungus-mediated bi-metallic Ag-Cu nanoparticles, Applied Science, 12 (2022) 1384-1387.
17. R. Singh and S. Dutta, Synthesis and characterization of solar photoactive TiO2 nanoparticles with enhanced structural and optical properties, Advanved Powder Technology, 29 (2017) 211-218.
18. S. Kumar, N. Bithel, S. Kumar, Kishan, M. Sen and C. Banerjee, Phyto-mediated synthesis of zinc oxide nanoparticles from Clerodendrum infortunatum L. leaf extract and evaluation of antibacterial potential, South African Journal of Botany, 164 (2024) 146-151.
19. R. Kaliyaperumal, K. Nagaraj, V. K. Poovan, K. Sakthikumar, C. Govindasamy and A. S. Sivakumar, Hydrothermal implementation with Zirconia: synthesis, characterization and investigation of biocidal activity of Ag/ZrO2 nanocomposites, Zeitschrift für Physikalische Chemie, 238 (2023).
20. S. K. Pradhan, V. Pareek, J. Panwar and S. Gupta, Synthesis and characterization of ecofriendly silver nanoparticles combined with yttrium oxide (Ag-Y2O3) nanocomposite with assorted adsorption capacity for Cu(II) and Cr(VI) removal: A mechanism perspective, Journal of Water Process Engineering, 32 (2019) 100917-100932.
21. J. Samuel, J. E. shaji, S. S. J. Dhas, S. Suresh, V. S. Vinita and C.S.Biju, UV-blocking performance and antibacterial activity of Cd, Ba co-doped ZnO nanomaterials prepared by a facile wet chemical method, Surface and Interface Analysis, 1 (2023) 1-14.
22. C. J. C. Singh, J. Samuel, C. S. Biju, S. S. J. Dhas and S. Usharani, Effect of Sn doping on the structural, photoluminescence, ultraviolet fltering and antibacterial activity of ZnO nanorods, Optical and Quantum Electronics, 55 (2023) 1072-1093.
23. R. Yogamalar, R. Srinivasan, A. Vinu, K. Ariga and A.C. Bose, X-ray peak broadening analysis in ZnO nanoparticles, solid State Communication, 149 (2009) 1919-1923.
24. H. M. Shinde, S. V. Kite, B. S. Shirke and K. M. Garadkar, Eco-friendly synthesis of Ag-ZrO2 nanocomposites for degradation of methylene blue, Journal of Mater Science: Materials in Electronics, 32 (2021) 14235–14247.
25. J. Samuel, T.S. F. Rajesh, C.S. Biju, S. S. J. Dhas and S. Usharani, Synthesis, structural, photoluminescence, ultraviolet blocking and antibacterial performances of Ba-doped ZnO nanostructures, Results in Optics, 12 (2023) 100482-100489.
26. A. Bumajdad, A. A. Nazeer, F. A. Sagheer, S. Nahar and M. I. Zaki, Controlled Synthesis of ZrO2 Nanoparticles with Tailored Size, Morphology and Crystal Phases via Organic/Inorganic Hybrid Films, Scientific Report, 8 (2018) 3695-3704.
27. J. Samuel, S. Suresh, S. Shabna, V. S. Vinita, N. J. Ananth, P. M. S. Shinu, A. Mariappan, T. Simon, Y. Samson and C.S. Biju, Characterization and antibacterial activity of Ti doped ZnO nanorods prepared by hydrazine assisted wet chemical route, Physica E Low Dimensions System and Nanostructures, 143 (2022) 115374-115381.
28. S. Iqbal, M. Javed, A. Bahadur, M.A. Qamar, M. Ahmad, M. Shoaib, M. Raheel, N. Ahmad, M.B. Akbar and H. Li, Controlled synthesis of Ag doped CuO nanoparticles as a core with poly(acrylic acid) microgel shell for efcient removal of methylene blue under visible light, Journal of Materials Science: Materials in Electronics, 31 (2020) 8423-8432.
29. J. Fanga and Y. Xuan, Investigation of optical absorption and photothermal conversion characteristics of binary CuO/ZnO nanofluids, RSC Advances, 7 (2017) 56023-56028.
30. H, Huang, X. Sun, S. Wang, Y. Liu, X. Li, J. Liu, Z. Kang and S. T. Lee, Strong red emission of pure Y2O3nanoparticles from oxygen related defects, Dalton Transactions, 40 (2011) 11362-11366.
31. M. Mohammadikish and A.A. Akradi, Synthesis and optical band gap determination of CuO nanoparticles from salen-based infinite coordination polymer nanospheres, Material Research Express, 6 (2019) 045013.
32. J. Samuel, J. E. shaji, S. S. J. Dhas, S. Suresh, V. S. Vinita and C.S.Biju, UV-blocking performance and antibacterial activity of Cd, Ba co-doped ZnO nanomaterials prepared by a facile wet chemical method, Surface and Interface Analysis, 1 (2023) 1-5.
33. A. Ravi, J. Samuel, S. S. J. Dhas, S. Usharani, T. Simon, D. S. Kumar, S. K. J. Vijitha, A. Sivakumar, R. S. Kumar, C. S. Biju, Structural, morphological, optical and antibacterial performances of rare earth (Sm)-doped ZnO nanorods, Journal of Rare Earths, (2024) In press.
34. A. Pramothkumar, N. Senthilkumar, K.C.M. Gnana Malar, M. Meena and I.V. Potheher, A comparative analysis on the dye degradation efciency of pure, Co, Ni and Mn doped CuO nanoparticles, Journal of Materials Science: Materials in Electronics, 30 (2019) 19043-19047.
35. S. Balaji, B. K. Mandal, S. Ranjan, N. Dasgupta and R. Chidambaram, Nano-zirconia – Evaluation of its antioxidant and anticancer activity, Journal of Photochemistry and Photobiology B: Biology, 170 (2017) 125-133.
36. A. K. Chitoria, A. Mir and M.A. Shah, A review of ZrO2 nanoparticles applications and recent advancements, Ceramics International, 49 (2023) 32343-32358.
37. S. Prabhu, T.D. Thangadurai, P.V. Bharathy, Greenbased Biosynthesis of Zinc Oxide Nanoparticles Using Clitoriaternatea Flower Extract and Its Antibacterial Activity, Nano Biomedical Engineering, (2021) 394-400.
38. M. E. T. Yazdi, M. S. Amiri, S. Akbari, M. Sharifalhoseini, F. Nourbakhsh, M. Mashreghi, M. R. E. Yousefi, M. Abbasi, A. H. Modarres, Green synthesis of silver nanoparticles using helichrysum graveolens for biomedical applications and wastewater treatment, Bio. NanoScience, 10 (2020) 1121–1127.
39. M. J. Akhtar, H.A. Alhadlaq, A. Alshamsan, M.A. Majeed Khan and M. Ahamed, Aluminum doping tunes band gap energy level as well as oxidative stress-mediated cytotoxicity of ZnO nanoparticles in MCF-7 cells, Scientific Reports, 5 (2015) 13876.
40. M. Ahamed, M. J. Akhtar, M. A. M. Khan and H. A. Alhadlaq, Enhanced Anticancer Performance of Eco-Friendly-Prepared MoZnO/RGO Nanocomposites: Role of Oxidative Stress and Apoptosis, ACS Omega, 7 (2022) 7103.
41. R. Rajendran and A. Mani, Photocatalytic, antibacterial and anticancer activity of silver-doped zinc oxide nanoparticles, Journal of Saudi Chemical Society, 24 (2020) 1010–1024.
42. N. Lewinski, V. Colvin and R. Drezek, Cytotoxicity of Nanoparticles, small, 4 (2008) 26–49.
How to Cite
H. R. Uma, & Jessica Fernando. (1). Green synthesis, Characterization and Anticancer activityof Clerodendrum infortunatum mediated ZrO2 and Ag doped ZrO2 nanoparticles. Revista Electronica De Veterinaria, 25(2), 2460-2470. https://doi.org/10.69980/redvet.v25i2.2263
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