ENDOPHYTIC FUNGAL DIVERSITY AND PHYTOCHEMICAL LOCALIZATION IN THREE MEDICINAL PLANTS FROM PHANSAD WILDLIFE SANCTUARY, RAIGAD, MAHARASHTRA
Keywords:
Endophytic fungi, Secondary metabolites, Histochemical analyses and Pharmacological properties.
Abstract
Endophytic fungi are a prolific and underexplored source of bioactive secondary metabolites with promising pharmacological and agricultural applications. This study aimed to isolate and characterize the endophytic fungi from three ethnomedicinally important plants Azadirachta indica (Neem), Centella asiatica (Gotu kola or Indian pennywort), and Abrus precatorius (Rosary pea) were collected from "Phansad Wildlife Sanctuary, Murud and Roha talukas of Raigad district, Maharashtra state, India". Standard isolation protocols using surface sterilization and culture on potato dextrose agar (PDA) were employed. Isolated fungal strains were morphologically identified using light microscopy and literature keys. Total nine number of Endophytic fungi were isolated from above three plants. Crude extracts of fungal cultures and host plants were subjected to Soxhlet extraction using ethyl acetate and subsequently screened for the presence of major phytochemicals including alkaloids, flavonoids, phenols, tannins, and proteins through qualitative assays and UV-Visible spectrophotometry. Histochemical and Microtomy staining techniques were utilized to observe tissue-level localization of these metabolites. The results highlight the presence of bioactive compounds in all three plant species, with significant accumulation in treated samples compared to controls. This highlights the potential role of endophytes in enhancing the phytochemical profile of their host plants. These findings support the hypothesis that endophytic fungi may play a crucial role in the regulation and biosynthesis of secondary metabolites, which could be harnessed for industrial and therapeutic purposes.
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How to Cite
Rafi Ahmed, Rukhsar Bano Ansari, & Sane Khadija. (1). ENDOPHYTIC FUNGAL DIVERSITY AND PHYTOCHEMICAL LOCALIZATION IN THREE MEDICINAL PLANTS FROM PHANSAD WILDLIFE SANCTUARY, RAIGAD, MAHARASHTRA. Revista Electronica De Veterinaria, 25(2), 2491-2510. https://doi.org/10.69980/redvet.v25i2.2275
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