Synergistic Integration of Collagen-Gel Bioactive Glass (Col-gel-BG) Scaffolds with Autologous Platelet-Rich Fibrin (PRF) for Enhanced Osteoconduction and Accelerated Bone Tissue Regeneration.

Prateek Veerendrakumar Siddhapur; Dr. Dinesh kumar

doi:10.69980/redvet.v25i2.2397

Prateek Veerendrakumar Siddhapur
Dr. Dinesh kumar

DOI: https://doi.org/10.69980/redvet.v25i2.2397

Keywords: Bioactive Glass; Collagen; Platelet-Rich Fibrin; Bone Tissue Engineering; Synergistic Regeneration.

Abstract

Background: The regeneration of critical-sized bone defects remains a significant clinical challenge. While bioactive glass and collagen scaffolds offer osteoconductive properties, they often lack the potent biological cues required for accelerated healing. This study investigates the synergistic effect of integrating Collagen-Gel Bioactive Glass (Col-gel-BG) scaffolds with autologous Platelet-Rich Fibrin (PRF).

Methods: Col-gel-BG scaffolds (70:30 ratio) were synthesized via lyophilization. Thirty New Zealand White rabbits were divided into three groups (n=10): Control (empty defect), Scaffold Only (Col-gel-BG), and Composite (Col-gel-BG + PRF). A 5 mm segmental radial defect was created in each animal. Bone regeneration was evaluated at 4, 8, and 12 weeks using radiography, Micro-Computed Tomography (Micro-CT), and histomorphometry.

Results: At 12 weeks, the Composite group exhibited significantly higher Bone Volume/Total Volume (62.18% ± 5.82%) compared to the Scaffold Only (38.62% ± 4.55%) and Control (12.45% ± 2.12%) groups (p < 0.05). Radiographic scoring indicated significantly earlier mineralization in the Composite group as early as 4 weeks. Histological analysis revealed mature lamellar bone formation and accelerated scaffold turnover in the presence of PRF, with significantly less residual material (7.12%) compared to the scaffold-only group (18.44%).

Conclusion: The integration of PRF into Col-gel-BG scaffolds creates a bioactive microenvironment that transitions the healing process from passive osteoconduction to accelerated osteogenesis. This autologous composite approach represents a promising advancement in bone tissue engineering for orthopedic and maxillofacial applications.

Author Biographies

Prateek Veerendrakumar Siddhapur

B.D.S-Undergraduate Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, India.

Dr. Dinesh kumar

Assistant professor, Department of Pedodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical sciences (SIMATS), Chennai – 600077 India

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