Invitro Antibacterial Activity and GC-MS Analysis of Acalypha Fruticosa Leaf Extract Against Some Human Pathogens
Keywords:
Antimicrobial Activity, Enterobacter aerogens, Acalypha fruticosa, GC-MS analysis.
Abstract
Medicinal plants are of special interest to biotechnology researchers because most of the drug industry uses them to make pharmaceutical compounds. Most herbal medications and their derivative products have crude plant extracts as their main constituent, which are a complex mixture of several phytochemical substances (plant secondary metabolites). The chemical properties of these constituents differ substantially among species. The findings showed that the plant extracts affected all of the bacteria that were utilised. In the current investigation, which extracts the leaves from Acalypha fruticosa using various solvents, ethyl acetate showed high antibacterial activity against both Gram-positive and Gram-negative bacterial strains, in contrast to other solvent extracts. An intriguing technique for determining the concentration of certain active ingredients in herbs used in cosmetics, medications, and pharmaceuticals is the GC-MS method, which is employed to analyse the extracted materials.
References
1. Bakal SN, Bereswill S, Heimesaat MM. Finding novel antibiotic substances from medicinal plants—Antimicrobial properties of Nigella sativa directed against multidrug-resistant bacteria. Eur J Microbiol Immunol. 2017;7(1):92–8. 10.1556/1886.2017.00001 [DOI] [PMC free article] [PubMed] [Google Scholar]
2. Dejani N.N., Elshabrawy H.A., Bezerra Filho C.d.S.M., de Sousa D.P. Anticoronavirus and immunomodulatory phenolic compounds: Opportunities and pharmacotherapeutic perspectives. Biomolecules. 2021;11:1254. doi: 10.3390/biom11081254. [DOI] [PMC free article] [PubMed] [Google Scholar]
3. Despande R R, Kale A A, Rujikar A D, Panvalkar P S, Kulkarni A A, Despande N R and Salvekar J P (2011) Antimicrobial Activity of Different Extracts of Juglans regia L. Against Oral Microflora, Int J Pharm Pharm Sci, 3(2): 200-201.
4. Duraiarasan Surendhiran, Jayachandran Karthiga, Sekar Nirmala, Abdul Razack Sirajunnisa (2011) Isolation of Genistein from Acalypha fruticosa and Studying its Antibacterial Activity by Inhibition of Bacterial DNA and Protein, Journal of Herbal Medicine and Toxicology, 5(1): 87-96.
5. Duraipandiyan V, Ayyanar M and Ignacimuthu S (2006) Antimicrobial Activity of Some Ethnomedicinal Plants used by Paliyar Tribe from Tamil Nadu India, BMC Complement Altern Med,17: 6–35.
6. Fair RJ, Tor Y. Antibiotics and bacterial resistance in the 21st century. Perspect Medicin Chem
7. [Internet]. 2014;(6):25–64. Available from: 10.4137/PMC.S14459 [DOI] [PMC free article] [PubMed] [Google Scholar]
8. Falcone M, Paterson D. Spotlight on ceftazidime/avibactam: A new option for MDR Gram-negative infections. J Antimicrob Chemother. 2016;71(10):2713–22. 10.1093/jac/dkw239 [DOI] [PubMed] [Google Scholar]
9. Farell, Matteo Bassetti, Louise, Dembry M, Ptricia and Vincent T Andrilode (2002) Antimicrobial Agent and Chemotheraphy, 46.
10. Gupta M, Mazumdar U K, Sivahkumar T, Vamis M L M, Karki S, Sambathkumar R and Manikandan L, (2003) Antioxidant and Anti-inflammatory Activities of Acalypha fruticosa, Nigerian Journal of Natural Products and Medicine,7: 25–29.
11. Jayaweera D (1981) Medicinal Plants (Indigenous and Exotin) used in Ceylon, National Council of Srilanka, 2: 42-43.
12. Lautié E., Russo O., Ducrot P., Boutin J.A. Unraveling plant natural chemical diversity for drug discovery purposes. Front. Pharmacol. 2020;11:397. doi: 10.3389/fphar.2020.00397. [DOI] [PMC free article] [PubMed] [Google Scholar]
13. Laxminarayan R. The overlooked pandemic of antimicrobial resistance. Lancet. 2022;399:606–607. doi: 10.1016/S0140-6736(22)00087-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
14. Monajjemi M, Ilkhani A R, Nurul Aminin A L, Eghdami A, Mollaamin F and Rezaei S (2012) High-Performance Liquid Chromatography (HPLC) Nano Analysis of Antioxidant Compounds of Iranian Medicinal plants, Journal of Medicinal Plants Research ,6(18): 3459-3463
15. Mothana, Salah A A Abdo, Sidgi Hasson, Faisal M N Althawab, Sama A Z Alaghbari and Ulrike Lindequist (2010) Antimicrobial, Antioxidant and Cytotoxic Activities and Phytochemical Screening of Some Yemeni Medicinal Plants, Advance Access Publication, 7(3):323–330.
16. Phyllis Brown, Kathryn DeAntonois (1997) Handbook of Instrumental Techniques for Analytical Chemistry, High Performance Liquid Chromatography, 147-164.
17. Poulakou G, Lagou S, Karageorgopoulos DE, Dimopoulos G. New treatments of multidrug-resistant Gram-negative ventilator-associated pneumonia. Ann Transl Med. 2018;6(20):423–423. 10.21037/atm.2018.10.29 [DOI] [PMC free article] [PubMed] [Google Scholar]
18. Shriram V, Khare T, Bhagwat R, Shukla R, Kumar V. Inhibiting bacterial drug efflux pumps via phytotherapeutics to combat threatening antimicrobial resistance. Front Microbiol. 2018;9(DEC):1–18. 10.3389/fmicb.2018.00001 [DOI] [PMC free article] [PubMed] [Google Scholar]
19. Shubashini K Sripathi and Uma Sankari (2010) Ethnobotanical Documentationof a Few Medicinal Plants in the Agasthiayamalai Region of Tirunelveli District India, Ethnobotanical Leaflets 14: 173-81.
20. Subbarayan Gopalakrishnan, Krishnasami Saroja and Jeyaseelan Dulcy Elizabeth (2010) Wound Healing Activity of Acalypha fruticosa Forssk, Journal of Pharmacy Research, 3(9):2190-2191.
21. Thambiraj J and Paulsamy S (2011) Antimicrobial Screening of Stem Extract of the Folklore Medicinal Plant, Acalypha fruticosa Forssk, International Journal of Pharmacy and Pharmaceutical Sciences, 3(4):85-287.
22. Touati, N. et al. Antibacterial activity of phenolic compounds of Pulicaria odora, wild plant in northern Algeria. Int. Food Res. J. 25(5), 2121–2130 (2018).
2. Dejani N.N., Elshabrawy H.A., Bezerra Filho C.d.S.M., de Sousa D.P. Anticoronavirus and immunomodulatory phenolic compounds: Opportunities and pharmacotherapeutic perspectives. Biomolecules. 2021;11:1254. doi: 10.3390/biom11081254. [DOI] [PMC free article] [PubMed] [Google Scholar]
3. Despande R R, Kale A A, Rujikar A D, Panvalkar P S, Kulkarni A A, Despande N R and Salvekar J P (2011) Antimicrobial Activity of Different Extracts of Juglans regia L. Against Oral Microflora, Int J Pharm Pharm Sci, 3(2): 200-201.
4. Duraiarasan Surendhiran, Jayachandran Karthiga, Sekar Nirmala, Abdul Razack Sirajunnisa (2011) Isolation of Genistein from Acalypha fruticosa and Studying its Antibacterial Activity by Inhibition of Bacterial DNA and Protein, Journal of Herbal Medicine and Toxicology, 5(1): 87-96.
5. Duraipandiyan V, Ayyanar M and Ignacimuthu S (2006) Antimicrobial Activity of Some Ethnomedicinal Plants used by Paliyar Tribe from Tamil Nadu India, BMC Complement Altern Med,17: 6–35.
6. Fair RJ, Tor Y. Antibiotics and bacterial resistance in the 21st century. Perspect Medicin Chem
7. [Internet]. 2014;(6):25–64. Available from: 10.4137/PMC.S14459 [DOI] [PMC free article] [PubMed] [Google Scholar]
8. Falcone M, Paterson D. Spotlight on ceftazidime/avibactam: A new option for MDR Gram-negative infections. J Antimicrob Chemother. 2016;71(10):2713–22. 10.1093/jac/dkw239 [DOI] [PubMed] [Google Scholar]
9. Farell, Matteo Bassetti, Louise, Dembry M, Ptricia and Vincent T Andrilode (2002) Antimicrobial Agent and Chemotheraphy, 46.
10. Gupta M, Mazumdar U K, Sivahkumar T, Vamis M L M, Karki S, Sambathkumar R and Manikandan L, (2003) Antioxidant and Anti-inflammatory Activities of Acalypha fruticosa, Nigerian Journal of Natural Products and Medicine,7: 25–29.
11. Jayaweera D (1981) Medicinal Plants (Indigenous and Exotin) used in Ceylon, National Council of Srilanka, 2: 42-43.
12. Lautié E., Russo O., Ducrot P., Boutin J.A. Unraveling plant natural chemical diversity for drug discovery purposes. Front. Pharmacol. 2020;11:397. doi: 10.3389/fphar.2020.00397. [DOI] [PMC free article] [PubMed] [Google Scholar]
13. Laxminarayan R. The overlooked pandemic of antimicrobial resistance. Lancet. 2022;399:606–607. doi: 10.1016/S0140-6736(22)00087-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
14. Monajjemi M, Ilkhani A R, Nurul Aminin A L, Eghdami A, Mollaamin F and Rezaei S (2012) High-Performance Liquid Chromatography (HPLC) Nano Analysis of Antioxidant Compounds of Iranian Medicinal plants, Journal of Medicinal Plants Research ,6(18): 3459-3463
15. Mothana, Salah A A Abdo, Sidgi Hasson, Faisal M N Althawab, Sama A Z Alaghbari and Ulrike Lindequist (2010) Antimicrobial, Antioxidant and Cytotoxic Activities and Phytochemical Screening of Some Yemeni Medicinal Plants, Advance Access Publication, 7(3):323–330.
16. Phyllis Brown, Kathryn DeAntonois (1997) Handbook of Instrumental Techniques for Analytical Chemistry, High Performance Liquid Chromatography, 147-164.
17. Poulakou G, Lagou S, Karageorgopoulos DE, Dimopoulos G. New treatments of multidrug-resistant Gram-negative ventilator-associated pneumonia. Ann Transl Med. 2018;6(20):423–423. 10.21037/atm.2018.10.29 [DOI] [PMC free article] [PubMed] [Google Scholar]
18. Shriram V, Khare T, Bhagwat R, Shukla R, Kumar V. Inhibiting bacterial drug efflux pumps via phytotherapeutics to combat threatening antimicrobial resistance. Front Microbiol. 2018;9(DEC):1–18. 10.3389/fmicb.2018.00001 [DOI] [PMC free article] [PubMed] [Google Scholar]
19. Shubashini K Sripathi and Uma Sankari (2010) Ethnobotanical Documentationof a Few Medicinal Plants in the Agasthiayamalai Region of Tirunelveli District India, Ethnobotanical Leaflets 14: 173-81.
20. Subbarayan Gopalakrishnan, Krishnasami Saroja and Jeyaseelan Dulcy Elizabeth (2010) Wound Healing Activity of Acalypha fruticosa Forssk, Journal of Pharmacy Research, 3(9):2190-2191.
21. Thambiraj J and Paulsamy S (2011) Antimicrobial Screening of Stem Extract of the Folklore Medicinal Plant, Acalypha fruticosa Forssk, International Journal of Pharmacy and Pharmaceutical Sciences, 3(4):85-287.
22. Touati, N. et al. Antibacterial activity of phenolic compounds of Pulicaria odora, wild plant in northern Algeria. Int. Food Res. J. 25(5), 2121–2130 (2018).
Published
2024-10-20
How to Cite
G. Friya, & Dr. P.A. Mary Helen. (2024). Invitro Antibacterial Activity and GC-MS Analysis of Acalypha Fruticosa Leaf Extract Against Some Human Pathogens. Revista Electronica De Veterinaria, 25(2), 2842 - 2848. https://doi.org/10.69980/redvet.v25i2.2480
Section
Articles
